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Production Bibliography

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Antibody Production Bibliography
Revised September 2003



Altshuler, G.L., D.M. Dziewulski, et al. (1986). Continuous hybridoma growth and monoclonal antibody production in hollow fiber reactors-separators. Biotechnology and Bioengineering 28(5):646-658, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: hybridoma culture, polysulfone hollow fiber membrane modules, hybridoma cell line.

Ambrosius, H. (1996). Developmental aspects of immunoglobulins and antibodies. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):10-14, ISSN: 0946-7785.
Descriptors: IgY, IgG, evolution, diversity, birds, mammals.

Amos, B., M. Al-Rubeai and A.N. Emery (August 1994). Hybridoma growth and monoclonal antibody production in a dialysis perfusion system. Enzyme and Microbial Technology 16(8):688-695 ISSN:0141-0229.
NAL call number: TP248.E5E565
Descriptors: hybridoma cells, perfusion culture, culture media, reactor, tubular membrane.

Andersen, P.S., H. Orum, and J. Engberg (1996). One step cloning of murine Fab gene fragments independent of IgH isotype for phage display libraries. Biotechniques 20(3):340-342, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: immunoglobulin fragment, antibody production, molecular cloning, polymerase chain reaction, gene library, bacteriophage, mice.

Andersen, P.S., A. Stryhn, et al. (1996). A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells. Proceedings of the National Academy of Sciences of the United States of America 93(5):1820-1824, ISSN:0027-8424.
NAL call number: 500 N21P
Descriptors: molecule complexes, novel approach, alternative to conventional hybridoma techniques, phage display technology, mice.

Bae, C.S., D.S. Yang, K.R. Chang, et al. (1998). Enhanced secretion of human granulocyte colony-stimulating factor directed by a novel hybrid fusion peptide from recombinant Saccharomyces cerevisiae at high cell concentration. Biotechnology and Bioengineering 57 (5):600-609, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: recombinant human granulocyte colony-stimulating factor (rhG-CSF), fed-batch cultures, culture broth, antibody-specific immunoreactive proteins, Escherichia coli.

Banik, G.G. and C.A. Heath (1995). Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture. Biotechnology and Bioengineering 48(3):289-300, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: hybridoma cell line H22, growth rate, cell density, metabolic parameters.

Baron, D. (October 1990). Industrial production of monoclonal antibodies [Industrielle Produktion monoklonaler Antikorper.] Naturwissenschaften 77(10):465-471, ISSN:0028-1042.
NAL call number: 474 N213
Descriptors: murine monoclonal antibodies, hybridomas, bioreactors, encapsulation technology, fermentation.

Barry, M.A., M.E. Barry, and S.A Johnston (1994). Production of monoclonal antibodies by genetic immunization. Biotechniques 16(4):616,618,620, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: mice, genetic immunization, human growth hormone, hybridoma cells, biolistic transfection.

Batt, B.C., V.M. Yabannavar, and V. Singh (1995). Expanded bed adsorption process for protein recovery from whole mammalian, cell culture broth. Bioseparation 5(1):41-52, ISSN:0923-179X.
NAL call number: QH324.9.S4B56
Descriptors: Chinese hamster, ovary, cell culture, soluble protein, economical, comparison.

Baxter, R.C., S. Axiak, and R.L. Raison (February 1982). Monoclonal antibody against human somatomedin-C/insulin-like growth factor-I. Journal of Clinical Endocrinology and Metabolism 54(2):474-476, ISSN:0021-972X.
NAL call number: 448.8 J8294
Descriptors: IgG3, mouse hybridoma, hypopituitarism.

Behn, I. U. Hommel, M. Oertel, and S. Hauschildt (1996). Kinetics of IgY formation after immunisation of hens with different protein antigens. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):18-21, ISSN: 0946-7785.
Descriptors: IgY, protein antigen, titer, HRPO-labeling.

Bellei, N.C.J, C.F.H. Granato, and H. Tomiyama (1996). In vitro induced antibody production for the diagnosis of herpes simplex, virus infection in immunosuppressed patients. Brazilian Journal of Medical and Biological Research 29(4):485-487, ISSN:0100-879X.
NAL call number: R850.A1B72
Descriptors: test, herpes simplex virus, lymphocytes, Varicella zoster, human, specificity, diagnostic method.

Belloc, C., S. Baird S., et al. (1996). Human cytochromes p450 expressed in escherichia coli: production of specific antibodies. Toxicology 106(1-3):207-219, ISSN:0300-483X.
NAL call number: RA1190 T69
Descriptors: enzyme antibody, isoenzyme, antibody specificity, yeast, liver microsome, xenobiotic metabolism, human tissue.

Berchiche, Louiza (1995). Contribution to study of cell metabolism in culture and to improvement, of monoclonal antibody production by hybridoma cells.[Contribution a l'etude du metabolisme des cellules en culture et a, l'amelioration de la production d'anticorps monoclonaux par les hybridomes.] Universite de Nancy: Nancy, FRA, 270p.
Descriptors: thesis, human, monoclonal antibody, cell culture, hybridoma, culture medium, environmental factors, carnitine.

Berchiche, L., C. Legrand, et al. (May 15, 1994). Effect of L-carnitine and acylcarnitine derivatives on the proliferation and monoclonal antibody production of mouse hybridoma cells in culture. Journal of Biotechnology 34(2):175-183, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: mice, cell growth metabolism, antibody production, murine hybridoma cell line, fed harvest cultures, amino acid metabolism, tissue culture.

Bergwerff, A.A., C.J. Stroop, et al. (June 1995). Variation in N-linked carbohydrate chains in different batches of two chimeric monoclonal IgG1 antibodies produced by different murine SP2(0 transfectoma cell subclones. Glycoconjugate Journal 12(3):318-330, ISSN:0282-0080.
Descriptors: myeloma cell subclones, hollow fiber reactors, carbohydrate Sequence, multiple myeloma.

Bibila, T.A., and D.K. Robinson (January-February 1995). In pursuit of the optimal fed-batch process for monoclonal antibody production. Biotechnology Progress 11(1):1-13, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: large scale production, longevity, high specific antibody, culture parameters, cell line, culture media, hybridomas.

Bibila, T.A., and C.S. Ranucci (1994). Monoclonal antibody process development using medium concentrates. Biotechnology Progress 10(1):87-96, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: fed-batch process, biotechnology industry, cell culture longevity, antibody titers, purification method, analytical method, nutrition, metabolism.

Birch, J.R., J. Bonnerjea, S. Flatman, and S. Vranch (1995). The production of monoclonal antibodies. In: Monoclonal Antibodies: Principles and Applications J.R. Birch and E.S. Lennox, eds., Wiley Liss, Inc.: New York, NY, pp. 231-265, ISBN:0-471-05147-0.
Descriptors: animal cell culture, mammalian cells, manufacturing process, quality, price, monoclonal antibody, chromatographic methods.

Blankenstein, G., U. Spohn, F. Preuschoff, J. Thoemmes, and M.R. Kula (1994). Multichannel flow-injection-analysis biosensor system for on-line monitoring of glucose, lactate, glutamine, glutamate and ammonia in animal cell culture. Biotechnology and Applied Biochemistry 20(2):291-307, ISSN:0885-4513.
NAL call number: QD415.A1J63
Descriptors: hybridoma, cell culture, lactate, glutamate ammonia, flow-injection-analysis, bioreactor, computers.

Blasey, H.D., and U. Winzer (1989). Low protein serum-free medium for antibody production in stirred bioreactors. Biotechnology Letters 11(7):455-460, ISSN:0141-5492.
NAL call number: QR53 B56
Descriptors: monoclonal antibody, hybridoma, cell proliferation, mixed tank reactor, batch process,

Bliem, R., R. Oakley, et al. (November 1990). Antibody production in packed bed reactors using serum-free and protein-free medium. Cytotechnology 4(3):279-283, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: monoclonal antibodies, serum-free medium, culture media.

Bog-Hansen, T.C. (1995). Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose. Methods in Molecular Biology 45:177-81, ISSN:1064-3745.
NAL call number: QH506.M45
Descriptors: isolation, purification, culture media, immunosorbent techniques, mice.

Bohmann, A., R. Portner, H. Markl (October 1995). Performance of a membrane-dialysis bioreactor with a radial-flow fixed bed for the cultivation of a hybridoma cell line. Applied Microbiology and Biotechnology 43(5):772-780, ISSN:0175-7598.
NAL call number: QR1.E9
Descriptors: animal cells, toxic metabolites, cell culture, hybridomas, monoclonal biosynthesis, fermentation, glucose metabolism.

Bonnycastle, L.L.C., J.S. Mehroke, et al. (1996). Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage. Journal of Molecular Biology 258(5):747-762, ISSN:0022-2836.
NAL call number: 442.8 J8224
Descriptors: cysteine, polyclonal antibody, monoclonal antibody, protein, carbohydrate, antigen antibody reaction, phage display, antigen binding, bacteriophage, amino acid sequence.

Brennan, A., L. Denomme, D. Velez, and J.D. Macmillan (1987). A novel perfusion system for growth of shear-sensitive hybridoma cells and antibody production in a stirred reactor. Abstracts from the Annual Meeting of the American Society of Microbiologists 87:268.
Descriptors: cell growth, culture, biotechnology.

Brennan, A.J., J. Shevitz, and J.D. Macmillan (1987). A perfusion system for antibody production by shear sensitive hybridoma cells in a stirred reactor. Biotechnology Techniques 1(3):169-174, ISSN:0951-208X.
Descriptors: hybridoma cell line, flow filtration unit, bioreactor, culture medium, reactor.

Brennan A.J, Surette E.D., et al. (1988). A novel harvesting strategy to increase antibody productivity from a continuous hollow fiber bioreactor. Abstracts from the Annual Meeting of the American Society of Microbiologists 88:263.
Descriptors: biotechnology, feed rate, pH gradients, tissue culture.

Bruggemann, E.P., V. Chaudhary, M.M. Gottesman, and I. Pastan (1991). Pseudomonas exotoxin fusion proteins are potent immunogens for raising antibodies against p glycoprotein. Biotechniques 10(2):202-204,206,208-209, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: novel method, human P glycoprotein, Escherichia coli fusion, Pseudomonas exotoxin.

Bugarski, B., G.A. King, et al. (1989). Performance of an external loop air-lift bioreactor for the production of monoclonal antibodies by immobilized hybridoma cells. Applied Microbiology and Biotechnology 30(3):264-269, ISSN:0175-7598.
NAL call number: QR1.E9
Descriptors: hydrodynamic properties, mouse hybridoma cells, calcium alginate gel beads, alginate poly-L-lysine microcapsules.

Burnie, J.P., W. Brooks, et al. (May 1996). Defining antibody targets in Streptococcus oralis infection. Infection and Immunity 64(5):1600-1608, ISSN:0019-9567.
NAL call number: QR1.I57
Descriptors: in vitro, antigens, bacterial, Streptococcal infections, monoclonal antibodies, vaccines, epitopes.

Bushell, M.E., and S.L. Bell (1994). Enhancement of monoclonal antibody yield by hybridoma fed-batch culture, resulting in extended maintenance of viable cell population. Biotechnology and Bioengineering 44(9):1099-1106, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mice, growth rate, amino acids, biotechnology industry, nutrient consumption, maximum production rate.

Calcutt, M.J., A.A. Komissarov, M.T. Marchbank, and S.L. Deutscher (February 2, 1996). Analysis of a nucleic-acid-binding antibody fragment: construction and characterization of heavy-chain complementarily determining region switch variants. Gene 168(1):9-14, ISSN:0378-1119.
Descriptors: mice, inbred strains, filamentous bacteriophage, DNA binding proteins, immunoglobulin switch region, chimeric proteins.

Cartledge, C., C. Mclean, and J. Landon (1992). Production of polyclonal antibodies in ascitic fluid of mice: Time and dose relationships. Journal of Immunoassay 13(3):339-353, ISSN:0197-1522.
Descriptors: mice, intraperitoneal and subcutaneous immunization, Freund's complete adjuvant, Pristane, antibody titers, granuloma formation.

Cherian, P.V. and W.E. Magee (March 1990). Monoclonal antibodies to Chlamydia psittaci guinea pig inclusion conjunctivitis (GPIC) strain. Veterinary Microbiology 22(1):43-51, ISSN:0378-1135 .
Descriptors: guinea pig, conjunctivitis, hybridomas, IgG2A, IgG3.

Clemons, D.J., C. Besch-Williford, E.K. Steffen, L.K. Riley, and D.H. Moore (June 1992). Evaluation of a subcutaneously implanted chamber for antibody production in rabbits. Laboratory Animal Science 42(3):307-311, ISSN:0023-6764.
NAL call number:410.9 P94
Descriptors: polyclonal antibody, golf ball, titer quantity, comparison, Freund's, acylamide.

Cooper, P.D., C. McComb, and E.J. Steele (1991). The adjuvanticity of algammulin a new vaccine adjuvant. Vaccine 9(6):408-415, ISSN: 0264-410X.
NAL call number: QR189 V32
Descriptors: mice, immune stimulant, immunized comparison, Freund's complete adjuvant, alum preparation, in-vitro properties.

Cooper, P.D., E.J. Steele, et al. (1993). Gamma inulin and algammulin two new vaccine adjuvants. In: Vaccines: Modern Approaches to New Vaccines Including Prevention of Aids H.S. Ginsberg, et al., eds., Cold Spring Harbor Laboratory Press: Plainview, NY., pp. 25-30, ISBN:0-87969-383-5.
Descriptors: human suitability, mice, antigen, cell activation, high potency, low toxicity.

Cooper, P.D., R. Turner, and J. Mcgovern (1991). Algammulin gamma inulin alum hybrid adjuvant has greater adjuvanticity Than alum for hepatitis B surface antigen in mice. Immunology Letters 27(2):131-134, ISSN:0165-2478.
NAL call number: QR180 I53
Descriptors: mice, antibody titers, vaccine adjuvant, protein carrier.

Costantino, P.J., K.M. Franklyn, N.F. Gare and J.R. Warmington (1994). Production of antibodies to antigens of Candida albicans in CBA(H mice. Infection and Immunity 62(4):1400-1405, ISSN:0019-9567.
NAL call number: QR1.I57
Descriptors: Candida albicans, antibody production, immunoblotting, antibody detection.

Coy, M. and J.B. Neilands (1998). A new generation of antibodies. Journal of Applied Animal Welfare Science 1(2):103-117, ISSN:1088-8705.
NAL call number: IPSG9823
Descriptors: overview of uses and technology, hybirdoma, phage display, genetic engineering, alternative methods.

Coyle, P.V., D. Wyatt, C. McCaughey, and H.J. O'Neill (1992). A simple standardized protocol for the production of monoclonal antibodies against viral and bacterial antigens. Journal of Immunological Methods 153(1-2):81-84, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: one step footpad immunization, hybridoma, immunized mouse spleen, human respiratory virus, helicobacter pylori.

Croix, D.A., H.Y. Yeh, et al. (June 1993). A dominant epitope of HIV-1 protease recognized by hamster monoclonal antibodies. Journal of Acquired Immune Deficiency Syndrome 6(6):558-566, ISSN:0894-9255.
Descriptors: Armenian hamsters, hybridomas, immunodominant epitopes, antibody specificity.

Cutler, P., J. Bonnerjea, and C. Hill (1994). A comparison of methods for the purification of therapeutic grade monoclonal antibodies. Journal of Chemical Technology and Biotechnology 59 (1):110, ISSN:0268-2575.
NAL call number: TP1 J686
Descriptors: abstract, methods, purification method, biotechnology industry, pharmaceuticals, mammalian cell culture, protein A, chromatography, pH, isoelectric point.

Dandulakis, G., J.C. Herr, and D.J. Kirwan (September-October 1995). Cell growth and monoclonal antibody production in the presence of antigen and serum. Biotechnology Progress 11(5):518-524, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: hybridoma, cell cultures, cell line (ATCC TIB191), biotechnology methods, cattle, immunoconjugates, mice.

Darby, C.R., K. Hamano, and K.J. Wood (1993). Purification of monoclonal antibodies from tissue culture medium depleted of IGG. Journal of Immunological Methods 159(1-2):125-129, ISSN:0022-1759.
NAL call number: QR180 J6 .
Descriptors: tissue culture supernatant, IgG, hybridomas, rat, mouse, ascites, bovine fetal calf serum, gel purification method.

Davis, W.C. (1995). Methods in Molecular Biology, Vol. 45. Monoclonal antibody protocols. Humana Press Inc.: Totowa, NJ, USA, 264p., ISBN:0-89603-308-2.
Descriptors: antigens, heterohybridomas, stable bovine murine hybrids, rapid isotyping, bioreactors versus immune ascites, flow cytometry, enzyme binding.

De Deken, R., J. Brandt, et al. (February 1994). Influence of priming and inoculation dose on the production of monoclonal antibodies in two age groups of BALB(C) mice. Institute of Tropical Medicine Hybridoma 13(1):53-57, ISSN:0272-457X.
Descriptors: ascites, Taenia saginata cysticerci antigen, antibody titers, hybridomas, ascites, body weight, mice, Inbred BALB/c.

De Kruif, J., A.R. van der de Vries, et al. (1996). New perspectives on recombinant human antibodies. Immunology Today 17(10):453-455, ISSN:0167-5699.
Descriptors: phage display technology, B cells, in vitro, eukaryotic cells, immunotherapy.

Del Sorbo, G., A. Evidente, F. Scala (1994). Production of polyclonal antibodies for cyclopaldic acid, a major phytotoxic metabolite produced by the plant pathogen Seiridium cupressi. Natural Toxins 2(3):136-140, ISSN:1056-9014.
NAL call number: QP631 N37
Descriptors: rabbits, isolation, purification, antibody formation, binding.

Deo, Y.M., M.D. Mahadevan, and R. Fuchs (1996). Practical considerations in operation and scale-up of spin-filter based bioreactors for monoclonal antibody production. Biotechnology Progress 12(1):57-64, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: hybridoma, spin-filter configuration, perfusion bioreactors.

DeSilva, B.S. and G.S. Wilson (December 15, 1995). Solid phase synthesis of bifunctional antibodies. Journal of Immunological Methods 188(1):9-19, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: solid phase reactor, bismaleimide crosslinking reagent, goat, mouse, human, Fab fragements.

Doth, M. (1996). Assay development of a serum marker for myocardial injury: experiences with and avian anti-troponin I antibody. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):45-50, ISSN: 0946-7785.
Descriptors: comparison of chicken versus goat antibodies, diagnostic test, unspecific adherence.

Draber, P., E. Draberova, and M. Novakova (1995). Stability of monoclonal IgM antibodies freeze-dried in the presence of trehalose. Journal of Immunological Methods 181(1):37-43, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: disaccharide trehalose, mouse, monoclonal IgM antibodies, freeze-drying, storage, elevated temperatures, culture media, ascitic fluids.

Ducos, J., A.M. Bianchi-Mondain, et al. (January 1996). Hepatitis B virus (HBV) specific in vitro antibody production by peripheral blood mononuclear cells (PBMC) after vaccination by recombinant, hepatitis B surface antigen (rHBsAg). Clinical and Experimental Immunology 103(1):15-18, ISSN:0009-9104.
Descriptors: surface antigens, hepatitis B vaccines, synthetic vaccines immunology, cultured cells, recombinant proteins.

Duenas, M., A.C. Malmborg, et al. (1996). Selection of phage displayed antibodies based on kinetic constants. Molecular Immunology 33(3):279-285, ISSN:0161-5890.
NAL call number: 448.3 Im62
Descriptors: human antibodies, fab fragments, antigen chase, chemical coordination, analytical method.

Eggena, M., S.R. Targan, et al. (May 15, 1996). Phage display cloning and characterization of an immunogenetic marker (perinuclear anti-neutrophil cytoplasmic antibody) in ulcerative colitis. Journal of Immunology 156(10):4005-4011, ISSN:0022-1767.
NAL call number: 448.8 J8232
Descriptors: bacteriophage M13, cloning, autoantibodies isolation and purification, recombinant proteins.

Eldridge, J.H., J.K. Staas, et al. (1991). Biodegradable and biocompatible poly-dl-lactide-co-glycolide microspheres as an adjuvant for staphylococcal enterotoxin B toxoid which enhances the level of toxin-neutralizing antibodies. Infection and Immunity 59(9):2978-2986, ISSN:0019-9567.
NAL call number: QR1.I57
Descriptors: mice, vaccine delivery system, subcutaneously injection, IgG, antitoxin titers.

Eldridge, J.H., J.K. Staas, et al. (1993). New advances in vaccine delivery systems. Seminars in Hematology 30(4 Suppl. 4):16-25, ISSN:0037-1963.
Descriptors: literature review, monkey, simian immunodeficiency virus, Staphylococcal enterotoxin B, pulsed release.

Eldridge, J.H., J.K. Staas, et al. (1991). Biodegradable microspheres as a vaccine delivery system. Molecular Immunology 28(3):287-294, ISSN:0161-5890.
NAL call number: 448.3 Im62
Descriptors: mouse, toxoid, immunologic drug, Staphylococcal enterotoxin B, immunoglobulin G, intraperitoneal injection.

Emery, A.N., D.C.H. Jan, M. Al-Rubeai (1995). Oxygenation of intensive cell-culture system. Applied Microbiology and Biotechnology 43(6):1028-1033, ISSN:0175-7598.
NAL call number: QR1.E9
Descriptors: high-cell-density culture, spin filter perfusion, bench-top bioreactor cell growth inhibition,, monoclonal antibody production.

Erhard, M., P. Schmidt, et al. (1996). Production and purification of mouse IgG subclass specific chicken egg yolk antibodies using a new indirect affinity chromatography method with protein G sepharose. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):66-69, ISSN: 0946-7785.
Descriptors: advantages of chicken antibodies, White Leghorn, extraction of IgY, ELISA.

Eyer, K., A. Oeggerli, and E. Heinzle (1995). On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine. Biotechnology and Bioengineering 45(1):(54-62), ISSN: 0006-3592.
NAL call number: 381 J8224
Descriptors: animal cell cultures, hybridoma, cell line oxygen transport, mouse.

Falkenberg, F.W. (March 1994). Comparison of different in vitro culture methods. FORUM: Haraeus Instruments Laboratory Instruments Division 4-5.
Descriptors: hybridoma technology, monoclonal antibody production, conventional culture systems, dialysis membrane culture systems, hollow fiber culture systems, in vivo culture.

Falkenberg, F.W., Weichert, C., Krane, et al. (February 13, 1995). In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter. Journal of Immunological Methods 179(1):13-29, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: high density culture of hybridoma, dialysis membrane, comparison with in vitro methods.

Felici F., G. Galfre, et al. (1996). Phage-display peptides as tools for characterization of human sera. Methods in Enzymology 267:116-129, ISSN:0076-6879.
NAL call number: QP601 M49
Descriptors: monoclonal antibody, epitope, serum, antigen binding, antibody combining site, ligand binding, bacteriophage, bacteriophage M13, antigen detection.

Fischer, R.W. and P. C. Ferber (1992) Monoclonal antibodies: a comparison of in vitro production methods. [Monoklonale antikrper: in vitro produktionsmethoden im vergleich.] Alternativen zu Tierexperimenten: ALTEX 16:15-24, ISSN: 0946-7785.
Descriptors: cell culture, hen's egg, anitbody production.

Fitchen, J. (1994). Production of monoclonal antibodies and candidate vaccines in and plant cell cultures. In Vitro Cellular and Developmental Biology Animal 30A(3 PART 2):36, ISSN:1071-2690.
NAL call number: QH585 A1I58
Descriptors: natural product synthesis, pharmaceutical synthesis, synthetic method.

Fitchen, J., R.N. Beachy and M.B. Hein (August 1995). Plant-virus expressing hybrid coat protein with added murine epitope elicits autoantibody response. Vaccine 13(12):1051-1057, ISSN:0264-410X
NAL call number: QR189 V32
Descriptors: mice, C57BL/J, BALB/cBy, tobacco mosaic virus, zona pellucida, ZP3 epitope, vaccine production.

Forough, R., D. Hasenstab, et al. (1996). Generating antibodies against secreted proteins using vascular smooth muscle cells transduced with replication-defective retrovirus. Biotechniques 20(4):694-696, 698-701, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: alternative method, polyclonal antiserum baboon tissue rat arterial smooth muscle cells complementary DNA, gene expression.

Fu, P. and J.P. Barford (1994). Methods and strategies available for the process control and optimization of monoclonal antibody production. Cytotechnology 14(3):219-232, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: hybridoma cell culture, interactive environmental factors.

Galfre, G., P. Monaci, et al. (1996). Immunization with phage display mimotopes. Methods in Enzymology 267:109-115, ISSN:0076-6879.
NAL call number: QP601 M49
Descriptors: coat protein, virus antigen, virus antibody, bacteriophage, antigen recognition, cross reaction, mice.

Gao, J., M.F. Wahl, G. An, and J.M. Lee (1994). Enhancement of the monoclonal antibody production in genetically modified plant cell cultures. 207th National Meeting of the American Chemical Society, San Diego, California, USA, March 13-17, 1994. Abstracts of Papers from the American Chemical Society, ISSN:0065-7727.
Descriptors: meeting abstract, tobacco, immunologic method, synthetic method.

Geaugey, V. (1991). Membrane reactors for continuous production of monoclonal antibodies: kinetics, performances and indirect estimation of cell population. [Reacteurs membranaires pour la production continue d'anticorps monoclonaux:cinetique, performances et evaluation indirecte de la population cellulaire.] Institut National Polytechnique de Lorraine, Francee Univ., France, thesis, 260p.
Descriptors: hybridomas, monoclonal antibody production, membrane reactors, cell density.

Gebbert, A., M. Alvarez-Icaza, et al. (February 28, 1994). On-line monitoring of monoclonal antibody production with regenerable flow-injection immunosystems. Journal of Biotechnology 32(3):213-220, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: mice, hybridoma cells, perfusion reactor, enzyme linked immunosorbent assay.

George, A.J.T. and A.A. Epenetos (1996). Advances in antibody engineering. Expert Opinion on Therapeutic Patents 6(5):441-456, ISSN:1354-3776.
Descriptors: human, cloning, phage display technology, novel antibodies, recombinant molecules, diagnostic and therapeutic agents, patents, pharmacokinetics.

Gerl, M., C. Steinert, et al. (1996) Immunisation of chickens with the aminoterminal propeptide of bovine procollagen type III: specificity of egg yolk antibodies and comparison with immunoassays using rabbit and mouse antibodies. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):51-56, ISSN: 0946-7785.
Descriptors: radioimmunoassay, specificity, antifibrotic agents.

Germaschewski, V. and K. Murray (1996). Identification of polyclonal serum specificities with phage-display libraries. Journal of Virological Methods 58(1-2):21-32, ISSN:0166-0934.
NAL call number: QR355.J6
Descriptors: hepatitis B virus, synthetic peptide, epitope mapping, surface antigen, immune repertoire, macromolecules.

Gillette, R.W. (May 4, 1987). Alternatives to pristane priming for ascitic fluid and monoclonal antibody production. Journal of Immunological Methods 99(1):21-23, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: incomplete Freund's adjuvant, hybridoma cell inoculation, proteose peptone, thioglycollate, corn oil, mice, Inbred BALB/c.

Goodall, M. (October 1996). Production of monoclonal antibodies: In vivo versus in vitro methods. In: Second World Congress on Alternatives and Animal Use in the Life Sciences October 20-24, 1996, Utrecht, The Netherlands, J.B.F. van der Valk and L.F.M. van Zutphen, eds., Alternatives to Laboratory Animals: ATLA 24(Special Issue):117, ISSN:0261-1929.
NAL call number: Z7994.L3A53
Descriptors: abstract, yeasts, insect cells, bacteria, transgenic animals, bioreactors.

Goding, J.W. (1980). Antibody production by hybridomas. Journal of Immunological Methods 39:285-308, ISSN:0011-1759.
NAL call number: QR180 J6
Descriptors: immunization protocol, cell fusion protocol, cloning, animal model, myeloma cell.

Gorter, A., R.J. Van De Griend, et al. (1993). Production of bi-specific monoclonal antibodies in a hollow fibre bioreactor. Journal of Immunological Methods 161(2):145-150, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: therapeutic purposes, comparison with ascitic fluid, human immunoglobulin, isotype composition, synthetic method.

Graf, H., J.N. Rabaud, and J.M. Egly (February 1994). Ion exchange resins for the purification of monoclonal antibodies from animal cell culture. Bioseparation 4(1):7-20, ISSN:0923-179X.
NAL call number: QH324.9 S4B56
Descriptors: ascitic fluid, cell culture supernatant, isolation, cultured cells, hybridomas.

Grandics, P. (June 1994). Monoclonal antibody purification guide: Part 2. American Biotechnology Laboratory 12(7):12-14, ISSN:0749-3223.
NAL call number: TP248.13.A5
Descriptors: biotechnology methods, chromatography, culture media, hybridomas.

Graus, Y., M. De Baets, P. Van Breda Vriesman, and D. Burton (1996). Anti-acetylcholine receptor autoantibodies from thymus derived phage display antibody libraries of patients with myasthenia gravis. FASEB Journal 10(6):A1300, ISSN:0892-6638.
NAL call number: QH301.F3
Descriptors: human, genetics, peptides, amino acids.

Gray, F., J.S. Kenney, and J.F. Dunne (1995). Secretion capture and report web: Use of affinity derivatized agarose microdroplets for the selection of hybridoma cells. Journal of Immunological Methods 182(2):155-163, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: hybridoma cells, biosynthesis, flow cytometry, entrapment, detection, monoclonal antibody, isolation, purification.

Greenway, T., J.H. Eldridge, et al. (1994). Enhanced immune responses to VEE virus by immunization with a microsphere antigen vaccine. 94th General Meeting of the American Society for Microbiology, Las Vegas, Nevada, USA, May 23-27, 1994. Abstracts of the General Meeting Of the American Society for Microbiology 94:156, ISSN:1060-2011.
Descriptors: Venezuelan equine encephalitis virus, arbovirus, inactivated virus vaccine, mice, drug delivery system, adjuvant, serum antibody, mucosal antibody, challenge protection.

Gregoriadis, G. (1995). Engineering liposomes for drug delivery: Progress and problems. Trends in Biotechnology 13(12):527-537 ISSN:0167-7799.
NAL call number:TA166.T72
Descriptors: biotechnology methods, pharmaceuticals, drug delivery systems, human disease treatment, therapeutic method, vaccines, liposome technology.

Grimaldi, C.M. and D.L. French (1995). Monoclonal antibodies by somatic cell fusion. ILAR Journal 37(3):125-132, ISSN:0018-9960.
NAL call number: QL55 A1I43
Descriptors: antigenic specificity, hybiridoma, BALB/c mice, genetic engineering.

Haga, S., M. Nakagawa, et al. (August 1992). Purification of MPB70 and production of specific monoclonal antibodies. Hybridoma 11(4):483-492, ISSN:0272-457X.
NAL call number: QR185.8.H9H9
Descriptors: guinea pigs, delayed-type hypersensitivity, skin reaction, monoclonal antibodies, isolation and purification.

Handa-Corrigan, A., S. Nikolay, et al. (1995). Monoclonal antibody production in hollow fiber bioreactors: Process, control and validation strategies for manufacturing industry. Enzyme and Microbial Technology 17(3):225-230, ISSN:0141-0229.
NAL call number: TP248.E5E565
Descriptors: hollow fiber bioreactors, commercial manufacturing, monoclonal antibodies, mouse hybridoma.

Hanly, W.C., J.E. Artwohl, and B.T. Bennett (1995). Review of polyclonal antibody production procedures in mammals and poultry. ILAR Journal 37(3):93-118, ISSN:0018-9960.
NAL call number: QL55 A1I43
Descriptors: humoral and cellular immune response, adjuvants, injection route, species differences, rabbit, chicken, rodents, guinea pig, farm animals.

Hansen, H.A., B. Damgaard, and C. Emborg (1993). Enhanced antibody production associated with altered amino acid metabolism in a hybridoma high-density perfusion culture established by gravity separation. Cytotechnology 11(2):155-166, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: monoclonal biosynthesis, sedimentation based separator, cell flocculation.

Harigae, M., M. Matsumura, and H. Kataoka (May 31, 1994). Kinetic study on HBs-MAb production in continuous cultivation. Journal of Biotechnology 34(3):227-235, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: mice, hybridoma, T0405 cells, IgG monoclonal antibody, hepatitis B, culture media.

Hayashi, O., T. Katoh and Y. Okuwaki (1994). Enhancement of antibody production in mice by dietary Spirulina platensis. Journal of Nutritional Science and Vitaminology 40(50):431-441, ISSN:0301-4800.
NAL call number: 389.8 J825
Descriptors: mouse, interleukin 1, concanavalin A, phytohemagglutinin, immune response, diet.

Healey, D.G., D. Baker, S.E. Gschmeer, J.L. Turk (1987). An antigenic determinant common to lymphocytes and Langerhans cells of the guinea pig. International Archives of Allergy and Applied Immunology 82(2):120-124, ISSN:0020-5915.
Descriptors: mouse, anti-guinea pig monoclonal antibody, cell fusion.

Heath, C., R. Dilwith and G. Belfort (July 1990). Methods for increasing monoclonal antibody production in suspension and entrapped cell cultures: Biochemical and flow cytometric analysis as a function of medium serum content. Journal of Biotechnology 15(1-2):71-89, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: hybridoma, cell culture media, microporous hollow fiber reactors, cytology.

Heidemann, R., U. Riese, et al. (1994). The Super Spinner: A low cost animal cell culture bioreactor for the CO2 incubator. Cytotechnology 14(1):1-9, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: small quantities, monoclonal antibodies, polypropylene hollow fiber membrane, cell density, cultured cells analysis, hamsters, hybridomas.

Hein, M.B., Y. Tang, et al. (1991). Evaluation of immunoglobulins from plant-cells. Biotechnology Progress 7(5):455-461, ISSN:8756-7938.
NAL call number: TP248.2.B46.
Descriptors: Saccharomyces cerevisiae, tobacco plant, murine monoclonal antibody.

Hendriksen, C., J. Rozing, M.Van Der Kamp, and W. De Leeuw (1996). The production of monoclonal antibodies: Are animals still needed? Alternatives to Laboratory Animals: ATLA 24(1):109-110, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: monoclonal antibody production, laboratory animal, alternative method, in vitro.

Hengelage, F.W.T., M. Krane, et al. (1993). A simple and inexpensive high density dialysis tubing cell culture system for the in vitro production of monoclonal antibodies in high concentration. Journal of Immunological Methods 165:193-206, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: alternative to ascites production in mice, cell culture, cell media, oxygen consumption, hybridomas.

Hewish, D. (June 1996) Ascites and alternatives. ANZCCART News: Australian and New Zealand Council for the Care of Animals in Research and Teaching 9(2):8-10.
NAL call number: SF405.5 A3
Descriptors: mice, volume culture flasks, fermentors, in vivo versus in vitro methods.

Hill, H.R. and P.G. Stockley (1996). Phage presentation. Molecular Microbiology 20(4):685-692, ISSN:0950-382X.
NAL call number: QR74.M65
Descriptors: alternatives to hybridoma technology, novel peptide, vaccines, fusion phage, monoclonal antibody, vaccine development.

Hlinak, A., M. Krüger, et al. (1996). Studies on daignostic applications of egg yolk antibodies against Bordetella bronchiseptica. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):70-72, ISSN: 0946-7785.
Descriptors: polyclonal antibodies, mammalian alternative, immunohistochemistry.

Hlinak, A., W. Schördl, et al. (1996). Production of egg yolk antibodies against human cell-associalted antigens. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):76-79, ISSN: 0946-7785.
Descriptors: IgY, cell associated antigens, CD14, p23, alternative to mammalian antibodies.

Hofmann, A. M. Erhard, et al. (1996). Lipopeptides as adjuvants for the immunisation of laying hens.[ Lipopeptide als adjuvantien bei der immunisierung von legehennen.] Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):26-29, ISSN: 0946-7785.
Descriptors: lipopeptides, complete Freund's adjuvant, IgY, egg yolk.

Hofmann, F., W. Wrasidlo, D. deWinter, and S. Gallagher (1989) Fully integrated, compact membrane reactor system for the large scale production of monoclonal antibodies. In: Advances in Animal Cell Biology and Technology for Bioprocesses R.E. Spier, et al. (eds.), Butterworths: Seven Oaks, Kent, England, 585p., ISBN:0-407-01499-3
NAL call number: TP248.27 A53E97 1989
Descriptors: cell culture, biotechnology, cytology.

Holley, M.C. (1992). A simple in vitro method for raising monoclonal antibodies to cochlear proteins. Tissue Cell 24(5):613-624, ISSN:0040-8166.
NAL call number:QH573.T5
Descriptors: cochlear tissue, hybridoma supernatents, cell culture.

Holzmann, D. (September 15, 1994). Agracetus grows monoclonals in soybeans and corn plants. GEN: Genetic Engineering News 14(1):34 ISSN:0270-6377.
NAL call number: QH442 G456
Descriptors: DNA, transgenic seeds, colon cancer, herpes.

Hommel, U., I, Behn and S. Hauschildt (1996). IgY antibodies as secondary reagent in FACS analysis. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):40-44, ISSN: 0946-7785.
Descriptors: alternatives to mammalian antibodies, avian vittellin antibodies, IgY, labeling, FAC analysis.

Hsieh, C.S., S.E. Macatonia, A. O'Garra, and K.M. Murphy (February 1, 1995) T cell genetic background determines default T helper phenotype development in vitro. Journal of Experimental Medicine 181(2):713-721, ISSN:0022-1007.
NAL call number:448.8 J822
Descriptors: Leishmania major, T helper, in vitro, transgenic system, cultured cells, Inbred C57BL, mice.

Iijima, S., T. Mano, M. Taniguchi, and T. Kobayashi (1988). Immobilization of hybridoma cells with alginate and urethane polymer and improved monoclonal antibody production. Applied Microbiology and Biotechnology 28(6):572-576, ISSN:0175-7598.
NAL call number: QR1.E9
Descriptors: whole cell immobilization, gel strength, cell leakage prevention.

Irking, R.R., A.A. Kortt, and P.J. Hudson (1996). Affinity maturation of recombinant antibodies using E. coli mutator cells. Immunotechnology 2(2):127-143, ISSN:1380-2933.
Descriptors: genetic engineering, immunoglobulin fragment, phage display, binding affinity, Escherichia coli, recombinant DNA technology.

Jackson, L. R., L.J. Trudel, J.G. Fox and N.S. Lipman.(February 1999). Monoclonal Antibody Production in Murine Ascites I. Clinical and Pathologic Features. Laboratory Animal Science 49(1):70-80, ISSN:0023-6764.
NAL call number: 410.9 P94
Descriptors: ascites production, morbidity, mortality, animal welfare, in vivo, hybridoma cell lines, abdominal tap, tumor masses, pain.

Jackson, L. R., L.J. Trudel, J.G. Fox and N.S. Lipman.(February 1999). Monoclonal Antibody Production in Murine Ascites II. Production Characteristics. Laboratory Animal Science 49(1):80-86, ISSN:0023-6764.
NAL call number: 410.9 P94
Descriptors: ascites production, pristane, in vivo, hybridoma cell lines, abdominal tap, ascites volume, antibody concentration, production differences.

Jackson L.R., L.J. Trudel, J.G. Fox, and N.S. Lipman (1996). Evaluation of hollow fiber bioreactors as an alternative to murine ascites production for small scale monoclonal antibody production. Journal of Immunological Methods 189(2):217-231, ISSN: 0022-1759.
NAL call number: QR180 J6
Descriptors: bioreactor, hybridoma, hollow fiber membrane, ascites, enzyme linked immunosorbent assay, mouse.

Jakobovis, A. (October 1995). Production of fully human antibodies by transgenic mice. Current Opinion in Biotechnology 6(5):561-566, ISSN:0958-1669.
NAL call number:TP248.13 C87
Descriptors: monoclonal antibodies, human immunoglobulin loci, antigens.

Jaspert, R., T. Geske, et al. (January 13, 1995). Laboratory scale production of monoclonal antibodies in a tumbling chamber. Journal of Immunological Methods 178(1):77-87, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: hybridomas, dialysis tubes, monoclonal antibody synthesis, tissue culture.

Jeong, Y.H. and S.S. Wang (1995). Role of glutamine in hybridoma cell culture: effects on cell growth, antibody production, and cell metabolism. Enzyme and Microbial Technology 17(1):47-55, ISSN:0141-0229.
NAL call number: TP248.E5E565
Descriptors: mice, immunoglobulin G, antibody yield, monoclonal antibody production, glutamine consumption rate.

Jin, M., A. Larsson, and B.O. Nilsson (1996). Monoclonal antibodies against unfertilized zona-free mouse oocytes: characterization and effects on fertilization. Molecular Reproduction and Development 43(1):47-54, ISSN:1040-452X.
NAL call number: QP251 M64
Descriptors: mice, intrasplenic immunization, in-vitro fertilization, oocyte lysis, antigen localization, western blot.

Johnson, D.R. (1995). Murine monoclonal antibody development. Methods in Molecular Biology 51:123-137, ISSN:1064-3745.
NAL call number: QH506.M45
Descriptors: monoclonal biosynthesis, isolation, purification, ascites, cell fusion, clone cells, hamsters, hybridomas, immunization, mice, Inbred BALB/c, rats, cultured tumor cells.

Kameda, Y. and C. Hirota (February 1993). Production of a monoclonal antibody against cell-surface glycoprotein of guinea pig adrenocortical cells. Journal of Histochemistry and Cytochemistry 41(2):235-243, ISSN:0022-1554.
NAL call number: 381 J8222
Descriptors: guinea pig adrenal glands, cell-surface antigens, membrane glycoproteins.

Kamihira, M. (1994). Production and separation of useful biological substances in animal cell culture. Seibutsu-Kogaku Kaishi 72(5):379-390, ISSN:0919-3758.
NAL call number: QP601 A1N3
Descriptors: monoclonal antibody, hormones, cell immobilization, high cell density, growth rate, bioreactor, yield, productivity, metabolism, dissolved oxygen concentration.

Kamihira, M., H. Yoshida, S. Iijima, and T. Kobayashi (1990). Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma cell bioreactor. Journal of Fermentation and Bioengineering 69(5):311-312, ISSN:0922-338X.
NAL call number: QP601 A1J6
Descriptors: mouse, immobilization, alginates, polyurethane, cell culture, fluidized bed reactor, bioreactor, dilution rate.

Karu, A.E., C.W. Bell, and T.E. Chin (1995). Recombinant antibody technology. ILAR Journal 37(3):132-141, ISSN:0018-9960.
NAL call number: QL55 A1I43
Descriptors: structure, function, gene expression, engineering, semi-synthetic.

Kenney, J.S., F. Gray, M.H. Ancel, J.F. Dunne (1995). Production of monoclonal antibodies using a secretion capture report web. Biotechnology 13(8):787-790, ISSN:0733-222X.
Descriptors: production method, cell culture, hybridoma, flow cytometry.

Kerr, D.E., V.M. Vrudhula, H.P. Svensson, N.O. Siemers, and P.D. Senter (1999). Comparison of recombinant and synthetically formed monoclonal antibody-beta-lactamase conjugates for anticancer prodrug activation. Bioconjugate Chemistry10(6):1084-1089, ISSN:1043-1802.
NAL call number: QH345 B552
Descriptors: L49 monoclonal antibody, melanomas, carcinomas, Enterobacter cloacae beta-lactamase (bL), anticancer prodrugs, in vitro experiments, lung carcinoma cell lines, SN12P renal cell carcinoma tumors, nude, recombinant fusion protein, human tumor models.

Kim, J.H., and C.Y. Choi (1988). Effect of spent medium and stirring on the hybridoma growth and monoclonal antibody production in spinner flask and celligen bioreactor. Korean Journal of Applied Microbiology and Bioengineering 16(1):1-10, ISSN:0257-2389.
Descriptors: murine hybridoma, colon cancer, Chlamyida trachomatis, cell fusion, oxygen transfer, cell viability.

Kim, H.W. and D.S. Suh (1995). Study on the anti-hla antibody production using in vitro immunization technique. Korean Journal of Zoology 38(2):186-195, ISSN:0440-2510.
Descriptors: human anti-HLA monoclonal antibody production, splenocytes, congenic mice, thymocyte culture, macrophage, lymphocyte, immunoglobulin, elisa.

Kola, A., M.Baensch, et al. (1996). Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis. Immunotechnology 2(2):115-126, ISSN:1380-2933.
Descriptors: neutralizing antibody, anaphylatoxin, monoclonal antibody, synthetic peptide, epitope mapping, phage display inflammation.

Koningsberger, J.C., L. Wiegman, et al. (1996). Phage antibody display technology as novel tool to generate human antibodies against intestinal epithelial cells. Gastroenterology 110(4 Suppl.): A545, ISSN:0016-5085.
NAL call number: RC799 G37
Descriptors: gastrointestinal oncology, cancer therapy, cancer diagnosis, neoplastic cell lines, in vitro studies.

Koval'chuk, N.V., L.N. Chernousova, and M.V. Raevskaia (July-August 1995). Improving of methods of production and screening of monoclonal antibodies to Salmonella antigens. [Optimizatsiia metodov polucheniia i skrininga monoklonal'nykh antitel k sal'monelleznym antigenam.] Klinicheskaya Laboratornaya Diagnostika 4:39-41, ISSN:0869-2084.
Descriptors: bacterial antigens, ascitic fluid, antigens, salmonella, monoclonal biosynthesis, Inbred BALB/c, mice.

Kreuter, J., U. Berg, E. Liehl, M. Soliva, and P.P. Speiser (1986). Influence of the particle size on the adjuvant effect of particulate polymeric adjuvants. Vaccine 4(2):125-129, ISSN:0264-410X.
NAL call number: QR189 V32
Descriptors: mice, polymethyl methacrylate polystyrene, vaccine, antibody response.

Kreuter, J., E. Liehl, et al. (1988). Influence of hydrophobicity on the adjuvant effect of particulate polymeric adjuvants. Vaccine 6(3):253-256, ISSN:0264-410X.
NAL call number: QR189 V32
Descriptors: antibody response, bovine serum albumin, influenza virus.

Kromenaker, S.J. and F. Srienc (May-June 1994). Stability of producer hybridoma cell lines after cell sorting:a case study. Biotechnology Progress 10(3):299-307, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: mice, monoclonal biosynthesis cell division flow cytometry, fluorescent antibody technique.

Kuhlmann, I., W. Kurth, and I. Ruhdel (December 1989). Monoclonal antibodies: In vivo and in vitro production on a laboratory scale, with consideration of the legal aspects of animal protection. Alternative to Laboratory Animals: ATLA 17(2):73-82, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: mass production, laboratory scale production, vivo and in vitro methods, German animal protection law, animal regulations, Switzerland, Netherlands, animal testing alternatives, animal welfare.

Kuhlmann, I., W. Kurth, and I. Ruhdel (1989). Monoclonal antibodies: In vivo and in vitro production on a laboratory scale from the perspective of animal rights regulations. [Monoklonale antikoerper: In vivo und in vitro produktion im labormassstab unter beruecksichtigung tierschutzrechtlicher as.] Alternativen zu Tierexperimenten: ALTEX 11:12-26, ISSN: 0946-7785.
Descriptors: cell culture, regulations, alternative production methods.

Lacount, W., J.R. Ryland, P.M. Linzmaier, and J.M. Lee (1995). The stabilization of heavy chain monoclonal antibody produced in plant suspension culture. Abstracts of Papers from the American Chemical Society 209(1-2)BIOT 84, ISSN:0065-7727
Descriptors: Nicotiana tabacum, plant growth, rate.

Lang, I.M., C.F. III Barbas, and R.R. Schleef (1996). Recombinant rabbit Fab with binding activity to type-1 plasminogen activator inhibitor derived from a phage-display library against human alpha-granules. Gene 172(2):295-298, ISSN:0378-1119.
NAL call number: QH442 A1G4
Descriptors: antibody fragments, filamentous bacteriophage, rabbit antibody, clones, rabbit spleen, bone marrow purified human platelet, plasminogen activator, binding affinity.

Larrick, J.W. and J. Gavilondo (1996). Meeting report from the 'Therapeutic Antibody Technology 95' meeting: Not just another recombinant antibody meeting. Immunotechnology 2(1):59-63, ISSN:1380-2933.
Descriptors: genetic engineering, phage display, transgene, antibody structure.

Larsson A., R.M. Balow, T.L. Lindahl, and P.O. Forsberg (1993). Chicken antibodies: taking advantage of evolution, a review. Poultry Science 72(10):1807-1812, ISSN:0032-5791.
NAL call number: 47.8 AM33P
Descriptors: bird, poultry industry, immunoglobulin G.

Lee, Y.I., S.S. Surzycki, and Y.I. Lee (1995). Production of egg yolk antibody (IgY) against human placental, DNA-dependent RNA polymerase II. Journal of Biochemistry and Molecular Biology 28 (1):27-32, ISSN:1225-8687.
NAL call number: TP248.65 F66F66
Descriptors: polyclonal antibodies, human DNA dependent RNA polymerase II (HPP, II), chicken, immunoglobulin Y, gene isolation, transcription assay.

Lee, G.M. and B.O. Palsson (1994). Monoclonal antibody production using free-suspended and entrapped hybridoma cells. Biotechnology and Genetic Engineering Reviews 12:509-533, ISSN:0264-8725.
NAL call number: TP248.3 B46
Descriptors: monoclonal biosynthesis, hybridomas, immunology, cultured cells, tissue culture.

Lehmann, J., Buntemeyer, H., and V. Jager (1990). Bulk culture of animal cells for antibody production: A comparison of reactors. Food Biotechnology 4(1):423-431, ISSN:0890-5436.
NAL call number: TP248.65.F66F66
Descriptors: cell culture, antibodies, biosynthesis, laboratory methods.

Lerner, R.A., A.S. Kang, et al. (November 20, 1992) Antibodies without immunization. Science 258:1313-1314, ISSN: 0036-8075.
NAL call number: 470 SCI2
Descriptors: alternative method, Escherichia coli, combinatorial libraries.

Linardos, T.I., N. Kalogerakis, L.A. Behie, and L.R. Lamontagne (1992). Monoclonal antibody production in dialyzed continuous suspension culture. Biotechnology and Bioengineering 39(5):504-510, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mouse hybridoma, Celligen bioreactor, cell growth, cell viability.

Lindl, T. (1995). Development of human monoclonal antibodies. [Entwicklung humaner monoklonaler antikörper.] Alternativen zu Tierexperimenten: ALTEX 12:13-23, ISSN: 0946-7785.
Descriptors: in vitro immunization, somatic cell fusion, Epstein Barr virus transformation, human immunolglobulin gene libraries, phage display techniques.

Liu, Y. and W. Xu (1995). Comparative study on early and rapid method for production of rat monoclonal antibodies in vitro. Xi'an Yike Daxue Xuebao 16(3):324-326, ISSN:0258-0659.
Descriptors: hemorrhagic fever, renal syndrome virus, immunoglobulin, hybridoma cell culture, synthetic methods.

Lonberg, N., L.D. Taylor, et.al (April 28, 1994). Antigen-specific human-antibodies from mice comprising 4 distinct genetic modifications. Nature 368(6474):856-859, ISSN:0028-0836.
NAL call number: 472 N21
Descriptors: mice, hybridoma cell lines, human IgM, IgG, Ig kappa.

Lösch, U. (1996). How do the antibodies get into the chcken egg? [Wie kommen die antikörper ins hühnerei?] Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):15-17, ISSN: 0946-7785.
Descriptors: chicken, IgY, IgG, IgA, IgY, yolk, yolk-sac, amnion, allantois.

Lowrey, D.M., K. Meslovich, et al. (May 1994). Monoclonal antibody production and purification using miniature hollow fiber cell culture technology. American Biotechnology Laboratory 12(6):16-17, ISSN:0749-3223.
NAL call number: TP248.13.A5
Descriptors: mice, monoclonal biosynthesis, cell line, electrophoresis, polyacrylamide gel, hybridomas.

Lundkvist, A. and B. Niklasson (1992). Bank vole monoclonal antibodies against Puumala virus envelope glycoproteins: Identification of epitopes involved in neutralization. Archives of Virology 126(1-4):93-105, ISSN:0304-8608.
NAL call number: 448.3 Ar23
Descriptors: antigen and epitope specificities, magnetic beads, hantavirus, fluorescent antibody technique.

Lygren, I., P.G. Burhol, and H.L. Waldum (January-March 1984). Production and evaluation of VIP antibodies in rabbits. Materia Medica Polona 16(1):26-29, ISSN:0025-5246.
Descriptors: avidity, specificity, vasoactive intestinal polypeptide, rabbits, gastrin, glucogon, insulin, secretin.

Ma, J.K.C. (1995). Antibody expression in plants. ACS Symposium Series: Antibody Expression and Engineering H.Y. Wang (ed.), 604:56-69, ISSN:0097-6156.
NAL call number: QD1.A45
Descriptors: bioreactors, large scale antibody production, antibody engineering.

Ma, J.K.C. and M.B. Hein (October 1995). Plant antibodies for immunotherapy. Plant Physiology 109(2):341-346, ISSN:0032-0889.
Descriptors: recombinant expression systems, cell culture techniques, protein assembly capabilities, bulk production, low cost.

Ma, J.K.C. and M.B. Hein (December 1995). Immunotherapeutic potential of antibodies produced in plants. Trends in Biotechnology 13(12):522-527, ISSN:0167-7799.
NAL call number: TA166.T72
Descriptors: plant hosts, inexpensive, plentiful antibody supply, literature review, transgenic plants, gene transfer.

Maenaka, K., M. Furuta, et al. (January 26, 1996). A stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti-HEL monoclonal antibody, HyHEL10. Biochemical and Biophysical Research Communications 218(3):682-687, ISSN:0006-291X.
NAL call number: 442.8 B5236
Descriptors: coliphages genetics, genetic vectors, muramidase chemistry, bacteriophage M13, Escherichia coli enzymology.

Mahana, W., and A. Paraf (1993). Mice ascites as a source of polyclonal and monoclonal antibodies. Journal of Immunological Methods 161(2):187-192, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: BALB/c mice, pristane, antigen, egg ovalbumin, ascitic fluid, synthetic method.

Maiorella, B.L., R. Ferris, et al. (1993). Evaluation of product equivalence during process optimization for manufacture of a human IgM monoclonal antibody. Biologicals 21(3):197-205, ISSN:1045-1056.
NAL call number: QH301 J68
Descriptors: in vitro cell culture, antibody titer, immunologic agent, pharmacokinetics, pharmaceutical synthesis, liquid chromatography, synthetic method.

Margni, R.A., I.M. Borel, et al. (July 1992). The proportion of symmetric and asymmetric IgG antibody molecules synthesized by a cellular clone (hybridoma) can be regulated by placental culture supernatants. Cellular Immunology 142(2):287-295, ISSN:0008-8749.
NAL call number: QR180 C4
Descriptors: pregnancy, placenta, asymmetric IgG antibodies, antibody isolation and purification, Inbred BALB/c mice.

Marks, J.D., H.R. Hoogenboom, et. al. (1991). By passing immunization: human antibodies from V-gene libraries displayed on phage. Journal of Molecular Biology 222: 581-597, ISSN:0022-2836.
NAL call number: 442.8 J8224
Descriptors: phage display technology, antibody fragments, alternative to rodents.

Marks, J.D., A.D. Griffiths, et al. (July 1992). By passing immunization: building high affinity human antibodies by chain shuffling. Biotechnology 10: 779:783, ISSN:0733-222X.
Descriptors: bacteriophage, in vitro, genes, human antibodies.

Martens, D.E, C.D. De Gooijer, E.C. Beuvery, and J. Tramper (1992). Effect of serum concentration on hybridoma viable cell density and production of monoclonal antibodies in CSTRS and on shear sensitivity in air-lift loop reactors. Biotechnology and Bioengineering 39(9):891-897, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: death rate, insect cells, methods, device, stirred tank bioreactor, gas flow rate.

Marx, P.A., R.W. Compans, et al. (1993). Protection against vaginal SIV transmission with microencapsulated vaccine. Science 260(5112):1323-1327, ISSN:0036-8075.
NAL call number: 470 Sci2
Descriptors: Simian immunodeficiency virus, macaque, human immunodeficiency virus, systemic priming, intramuscular plus oral route, biodegradable microspheres, drug delivery.

Marx, U., M.J. Embleton, R. Fischer, F.P. Gruber, et al. (1997). Monoclonal antibody production: The report and recommendations of ECVAM Workshop 23. Alternatives to Laboratory Animals: ATLA 25:121-137, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: ascites, pain, hybridoma technology, in vitro production, replacement, refinement, reduction, regulatory aspects.

Marx, U. and W. Merz (1995). In vivo and in vitro production of monoclonal antibodies: Bioreactors vs immune ascites. Methods in Molecular Biology 45:169-176, ISSN:1064-3745.
NAL call number: QH506.M45
Descriptors: monoclonal biosynthesis, ascites, cell culture instrumentation, hybridomas, mice, rats.

Mathiot, B., A. Perani, et al. (1993). Increase of hybridoma productivity using an original dialysis culture system. Cytotechnology 11(1):41-48, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: monoclonal antibody production, dialysis tubing comparison with ascites production, cost control.

Matsumura, M. and F.R.P. Jr. Nayve (1995). Effects of ammonium ion removal on growth and MAb production of hybridoma cells. Cytotechnology 18(1-2):35-50, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: mouse, hepatitis B surface antigen, perfusion culture, culture broth, cross flow filtration, membrane fouling, culture method, cryotechnology.

Mazzoni, I.E., E. Jaffe, and A.C. Cuello (1991). Production and immunocytochemical application of a highly sensitive and specific monoclonal antibody against rat dopamine-beta-hydroxylase. Histochemistry 96(1):45-50, ISSN:0301-5564.
NAL call number: QH613 H57
Descriptors: rats, hybridoma, mouse IgG1, brain cytology.

McArdle, J.E. (Winter 1997/1998). Alternatives to ascites production of monoclonal antibodies . Animal Welfare Information Newsletter 8(3-4):1-2,15-18, ISSN:1050-561X.
NAL call number: aHV4701 A952
Descriptors: history, ascites, in vitro, discomfort, pain, flasks, roller bottles, bioreactors.

McArdle, J.E., J.J. Reddington, G. Reddington, and H. Halsey (1994). An inexpensive alternative to ascites methods for small, medium and large scale monoclonal antibody production. In Vitro Toxicology 7(2):174, ISSN:0888-319X.
NAL call number: RM300 I55
Descriptors: human lymphocyte, immunologic method.

McGuill, M.W. and Rowan, A.N. (Winter 1989). Refinement of monoclonal antibody production and animal well-being. ILAR News 31(1):7-10, ISSN:0018-9960.
NAL call number: QL55.A1I43
Descriptors: mice, ascites tumors, pristane, distress.

McKinney, K.L., R. Dilwith, and G. Belfort (May 1995). Optimizing antibody production in batch hybridoma cell culture. Journal of Biotechnology 40(1):31-48, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: flow cytometry, surface antibody fluorescence, hybridoma cell lines ATCC HB124, anti-idiotypic biosynthesis, culture media, hybridomas, mice.

Meilhoc, E., K.D. Wittrup, and J.E. Bailey (1990). Influence of dissolved oxygen concentration on growth, mitochondrial function and antibody production of hybridoma cells in batch culture. Bioprocess Engineering 5(6):263-274, ISSN:0178-515X.
NAL call number: TP248.3.B563
Descriptors: dissolved oxygen, antibody, hybridoma, metabolism, cell proliferation, mixed tank reactor, cell culture.

Mendez, M.J., H. Abderrahim, M. Noguchi, et al. (March 20,1995). Analysis of the structural integrity of YACS comprising human-immunoglobulin genes in yeast and in embryonic stem-cells. Genomics 26(2):294-307, ISSN:0888-7543.
NAL call number: QH445.2 G45
Descriptors: mice, human monoclonal antibody production, cloning, mouse stem cells, cell lines.

Mestecky, J. and J.H. Eldridge (1991). Targeting and controlled release of antigens for the effective induction of secretory antibody responses. Current Opinion in Immunology 3(4):492-495, ISSN:0952-7915.
NAL call number: QR180 C87
Descriptors: review, mouse, cellular immunity, vaccine, immunoglobulin A.

Moro, A.M., M.T. Rodrigues, et al. (November 1994). Multiparametric analyses of hybridoma growth on glass cylinders in a packed-bed bioreactor system with internal aeration. Serum-supplemented and serum-free media comparison for MAb production. Journal Immunological Methods 176(1):67-77, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: large scale production, bioreactor vessel, cost effective, culture media, hybridomas, affinity diffusion chambers.

Murray, K., K. Gull, and A.J. Dickson (1996). Dichloroacetate increases cell and antibody yields in batch cultures of a hybridoma cell lines. Biotechnology and Bioengineering 49 (4):377-382, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: pyruvate dehydrogenase, dichloroacetate, growth, metabolism, hybridoma cell line, laboratory scale, antibody yield.

Nagel, A., S. Koch, U. Valley, F. Emmrich, and U. Marx (1999). Membrane-based cell culture systems: an alternative to in vivo production of monoclonal antibodies. In: Alternatives to Animals in the Development and Control of Biological Products for Human and Veterinary Use: London Zoo, London U.K., September 24-26, 1998. Proceedings of a Symposium Organized by the International Association for Biologicals, the National Institute for Biological Standards and Control and the National Institute of Public Health and the Environment, Basel ; New York : S. Karger, 335 p., ISSN: 0301-5149, ISBN: 3-8055-6953-X (paper).
NAL call number: QR180.3 D4 v. 101
Descriptors: in vitro production, in vivo production, monoclonal antibodies (MAbs), Technomouse hybrid, bioreactor, membrane-based cell culture, ascites method, alternatives, disposable culture devices.

Naim, J.O. and C.J. Van Oss (1995). Silicone gels as adjuvants: Effects on humoral and cell-mediated immune responses. Zouhair Atassi, M. and G. S. Bixler, Jr., ed., In: Advances in, Experimental Medicine and Biology, Vol. 383: Immunobiology of Proteins and Peptides VIII: Manipulation or Modulation of the Immune Response Plenum Publishing Corp.: New York, NY, pp. 1-6, ISBN:0-306-45125-5.
Descriptors: rat, mice, mammary implant, antibodies, arthritis, inflammatory disease, bones, joints, fasciae, connective and adipose tissue pathology.

Nakamura, G.R., R. Byrn, et al. (November 1992). Monoclonal antibodies to the extracellular domain of HIV-1IIIB gp160 that neutralize infectivity, block binding to CD4, and react with diverse isolates. AIDS Research and Human Retroviruses 8(11):1875-1885, ISSN:0889-2229.
Descriptors: hamsters, CD4 immunology, HIV envelope protein gp120, antibody specificity.

Nakaoka, R., Y. Tabata, and Y. Ikada (1995). Enhanced antibody production through sustained antigen release from, biodegradable granules. Journal of Controlled Release 37(3):215-224, ISSN:0168-3659.
NAL call number: RS201 C64J68
Descriptors: mouse, intraperitoneal administration, ovalbumin, poly(DL-lactic acid) granules, IgG production, interleukin 1, in vitro.

Nayve, F.R.Jr., T. Misato, M. Matsumura, and H. Kataoka (May 31, 1994). HBs-MAb production in perfusion culture with selective ammonia removal system. Journal of Biotechnology 34(3):217-225, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: mice, serum-free perfusion cultures, hybridoma TO-405 cells, cell densities, perfusion cultures, cultured cells.

Nilson, B., B. Akerstrom, and L. Logdberg (May 1987). Cross reacting monoclonal anti-alpha 1-microglobulin antibodies produced by multi-species immunization and using protein G for the screening assay. Journal of Immunological Methods 99(1):39-45, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: BALB(c) mouse, hybridomas, alpha 1-microglobulin, monoclonal antibody biosynthesis.

Oh, D.J., S.K. Choi, and H.N. Chang (1994). High-density continuous cultures of hybridoma cells in a depth filter perfusion system. Biotechnology and Bioengineering 44(8):895-901, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: filter perfusion system, animal cell culture, monoclonal antibody yield, proteins.

Ohlin, M., H. Owman, M. Mach, and C.A. Borrebaeck (January 1996). Light chain shuffling of a high affinity antibody results in a drift in epitope recognition. Molecular Immunology 33(1):47-56, ISSN:0161-5890.
NAL call number: 448.3 Im62
Descriptors: human polyclonal and monoclonal antibodies, pathogens, toxins, hybridoma, coliphages, recombinant proteins.

Omasa, T., M. Kobayashi, et al. (1995). Enhancement of antibody production by growth factor addition in perfusion and hollow fiber culture systems. Biotechnology and Bioengineering 48(6):673-680, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: transferrin, bovine serum albumin (BSA), cell growth, hybridoma, perfusion culture.

Overkamp, D., S. Mohammed-Ali, C. Cartledge, and J. Landon (1988). Production of polyclonal antibodies in ascitic fluid of mice technique and applications. Journal of Immunoassay 9(1):51-68, ISSN:0197-1522.
Descriptors: BALB/c mice, intraperitoneal versus subcutaneously immunization, insulin, antibody titers.

Oyaas, K., T.E. Ellingsen, N. Dyrset, and D.W. Levine (1994). Hyperosmotic hybridoma cell cultures: increased monoclonal antibody production with addition of glycine betaine. Biotechnology and Bioengineering 44(8):991-998, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mouse, hybridoma cells, NaCl, sucrose, glycine betaine medium osmolality, physiology, titer, yield, osmoprotective compounds, stress.

Ozturk, S.S. and B.O. Palsson (November-December 1991). Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor. Biotechnology Progress 7(6):481-494, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: murine hybridoma cell line, cell growth, viability, cell density, energy metabolism.

Ozturk, S.S. and B.O. Palsson (1990). Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody production kinetics in continuous culture. Biotechnology Progress 6(6):437-446, ISSN:8756-7938.
NAL call number: TP248.2.B46
Descriptors: viability, ATP, amino acid, cell proliferation, production, continuous stirred tank reactor, cell culture.

Pakdel, F., F. Petit, et al. (1994). Over expression of rainbow trout estrogen receptor domains in Escherichia coli: characterization and utilization in the production of antibodies for immunoblotting and immunocytochemistry. Molecular and Cellular Endocrinology 104(1):81-93, ISSN:0303-7207.
NAL call number: QP187 A1M6
Descriptors: rabbits, rainbow trout, polyclonal antibodies, estrogen receptor, immunoblotting, antibody production.

Pakkanen, R. and M. Neutra (1994). Bovine colostrum ultrafiltrate: An effective supplement for the culture of mouse-mouse hybridoma cells. Journal of Immunological Methods 169(1):63-71, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: ultrafiltrate fraction, bovine colostrum, cell culture supplement, cultured mouse-mouse hybridomas, IgG, IgA antibodies.

Palmer, D.B., A.J. George, M.A. Ritter (July 1997). Selection of antibodies to cell surface determinants on mouse thymic epithelial cells using a phage display library. Immunology 91(3): 473-478, ISSN:0019-2805.
NAL call number: 448.3 IM6
Descriptors: monoclonal antibodies, epitopes, alternative approach, phage display library expressing single chain, mice, Inbred BALB C.

Pannell, R. and C. Milstein (1992). An oscillating bubble chamber for laboratory scale production of monoclonal antibodies as an alternative to ascitic tumors. Journal of Immunological Methods 146:43-48, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: hybridomas, in vitro, contaminating proteins, monoclonal antibodies.

Park, S.Y., and G.M. Lee (1995). Enhancement of monoclonal antibody production by immobilized hybridoma cell culture with hyperosmolar medium. Biotechnology and Bioengineering 48(6):699-705, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mice, hyperosmotic stress, S3H5-gamma-2bA2 hybridoma cells, hyperosmotic stress, product yield, antibody productivity.

Park, S.H. and D.D.Y. Ryu (1994). Cell cycle kinetics and monoclonal antibody productivity of hybridoma cells during perfusion culture. Biotechnology and Bioengineering 44(3):361-367, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mouse, perfusion culture, hybridoma cell line, cell cycle phases, culture condition, production optimization, synthetic method.

Perez, M.E., J. Gavilondo-Cowley, and M. Aguilar (1988). Evaluation of different culture conditions for the in-vitro propagation of the hybridoma ifi-b6-c5 and the production of monoclonal antibodies. Interferon y Biotecnologia 5(3):262-269, ISSN:0138-8878.
Descriptors: mouse, alternative, ascitic fluids, mass culture, bovine serum, human endothelial cell supernatant, roller cultures, cell density, immunoglobulin concentrations.

Persson, B. and C. Emborg (1992). A comparison of three different mammalian cell bioreactors for the production of monoclonal antibodies. Bioprocess Engineering 8(3-4):157-163, ISSN:0178-515X.
NAL call number: TP248.3.B563
Descriptors: stirred tank reactors, mammalian cell culture, hybridoma cell growth, monoclonal antibody production.

Peterson, N.C. (1996). Recombinant antibodies: alternative strategies for developing and manipulating murine-derived monoclonal antibodies. Laboratory Animal Science 46(1):8-14, ISSN:0023-6764.
NAL call number: 410.9 P94
Descriptors: protein interactions, therapeutic agents, monoclonal hybridoma production, computer assisted molecular modeling, in vitro techniques, phage display, reduce and replace animals.

Peterson, N.C. (Autumn 1999). In vitro antibodies and good-faith efforts: an overview of the NRC report on monoclonal antibody production. Lab Animal 5-8, ISSN:0093-7355.
NAL call number: QL55.A1L33
Descriptors: monoclonal antibody production (MAbs), ascites method, in vitro methods, alternatives, hybridoma, IACUC responsibility, in vivo production, pristine priming, NRC recommendations, rodent welfare.

Petrossian, A. and G.P. Cortessis (April 1990). Large-scale production of monoclonal antibodies in defined serum-free media in airlift bioreactors. Biotechniques 8(4):414-422, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: airlift bioreactors, hybridoma cell lines, serum free media, monoclonal isolation and purification.

Pilkington, G.R., L. Duan, et al. (1996). Recombinant human Fab antibody fragments to HIV-1 Rev and Tat regulatory proteins: Direct selection from a combinatorial phage display library. Molecular Immunology 33(4-5):439-450, ISSN:0161-5890.
NAL call number: 448.3 Im62
Descriptors: human Fab phage display library, peripheral blood lymphocytes, human immunodeficiency virus type-1, anti-Rev Fab clones.

Polson, A., M.B. Von Wechmar, G. Fazakerley (1980). Antibodies to proteins from yolk of immunized hens. Immunological Communications 9(5):495-514.
NAL call number:QR180 I425
Descriptors: IgY, hens, immunization, egg yolk.

Polson, A., M.B. Von Wechmar and M.H.V. Van Regenmortel (1980). Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications 9:474-493, ISSN:0090-0877.
NAL call number: QR180 I425
Descriptors: IgY, hens, immunization, egg yolk.

Porter, W.P., R.C. Quander and J.C. Rener (April 1990). Labored breathing in ascites-producing mice. Lab Animal 19(3):21-22, ISSN:0093-7355.
NAL call number: QL55 A1L33
Descriptors: female BALB/c, mice, ascites, production, hybridoma, pristane priming, adverse side-effects, necropsy.

Puhlmann, C.M. and F.A. Anderer (1995). Optimizing production of human monoclonal IgG antibodies by in vitro, primed human PBMC: influence of CD56+ NK cell depletion. Hybridoma 14(4):391-396, ISSN:0272-457X.
NAL call number: QR185.8.H9H9
Descriptors: human-mouse heterohybridomas, monoclonal antibody, IgG, interleukin 2, immunization, human cell.

Racher, A.J., D. Looby, and J.B. Griffiths (1990). Studies on monoclonal antibody production by a hybridoma cell line (C1E3). immobilised in a fixed bed, porosphere culture system. Journal of Biotechnology 15(1-2):129-146, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: cell culture, immobilized cell, optimization, glutamine, amino acid, fixed bed reactor, pore structure.

Ramirez, O.T., R. Mutharasan, and W.E. Magee (1987). A novel immobilized hybridoma reactor for the production of monoclonal antibodies. Biotechnology Techniques 1(4):245-250, ISSN:0951-208X.
NAL call number: TP248.24 B55
Descriptors: mouse, hybridoma cells, tubular reactor.

Rawat, M., P.R. Jatkar, and K.N. Sharma (1994) Monoclonal antibodies: production and potential uses in veterinary biotechnology. International Journal of Animal Sciences 9(1):85-93, ISSN:0970-2857.
Descriptors: cell hybridization, cell fusion Sendai viruses, tissue culture, mice, rats.

Reuveny, S., D. Velez, J.D. Macmillan, and L. Miller (1987). Factors affecting monoclonal antibody production in culture. Developments in Biological Standardization 66:169-175, ISSN:0301-5149.
NAL call number: QR180.3 D4
Descriptors: large scale production, stirred reactors, murine cell line.

Ried, J.L., M.K. Walker-Simmons, et al. (1992). Production of polyclonal antibodies in rabbits is simplified using perforated plastic golf balls. Biotechniques 12(5):660, 662, 664, 666, ISSN:0736-6205.
NAL call number: RB37 A1B56
Descriptors: surgically implanted, subcutaneously, stress, animal handling, high titered antibodies, alternative method.

Rieger, A., W. Bürger, et al. (1996). Determination of human serum CRP using a chicken egg yolk antibody (IgY) to avoid interferences with rheumatoid factors. Comparison with mammalian antibodies. [Bestimmung von humanem serum-CRP mit einem aviären antikörper (IgY) im vergleich mit einem antikörper vom säuger (IgG): Falsch positive befunde durch interferenz mit rheumafaktoren.] Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):57-61, ISSN: 0946-7785.
Descriptors: IgY, rheumatoid factors, comparison with mammalian antibodies.

Riese, U., D. Lutkemeyer, et al. (May 31, 1994). Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology 34(3):247-257, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: chromatography, ion exchange, culture media, cytological techniques, monoclonal biosynthesis, cell count, hybridomas, mice.

Ritter, M. A. and H. M. Ladyman (1995) Monoclonal Antibodies: Production, Engineering, and Clinical Application Cambridge University Press: Cambridge, England, UK, New York, NY, USA. pp. 429-466, ISBN:0-521-47354-3.
NAL call number: QR186.85 M6623 1995
Descriptors: human, immunoglobulin G, immunoglobulin, M, cell culture, contamination, cryopreservation, cloning techniques, screening.

Rowan, A.N. (1995). The third R: Refinement. Alternative to Laboratory Animals: ATLA 23:332-346, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: alternatives, distress, anxiety, suffering, antibody production.

Rudbach, J.A., J.L. Cantrell, and J.T. Ulrich. (1988). Molecularly engineered microbial immunostimulators. In: Technolological Advances in Vaccine Development. R. Alan (ed.), Liss, Inc.: NY, pp. 443-454, ISBN:0-845-12683-0.
NAL call number: QH506.U34 v.84
Descriptors: non-toxic, non-allergenic, in vitro synthesis, human use.

Rudbach, J.A., J.L. Cantrell, and J.T. Ulrich (1995). Methods of measuring antibodies in sera plasma, ascites, and tissue culture media. Methods in Molecular Biology 45:79-82, ISSN:1064-3745.
NAL call number: QH506.M45
Descriptors: mice, hybridomas, elisa, monoclonal antibody, anti-immunoglobulin, immunologic method, analytical method.

Ruhdel, I. and I. Kulhlmann (1994). Production of monoclonal antibodies in roller bottles with reduced serum concentrations and serum substitutes. Bioengineering 10(2):15-18, 21-22, ISSN:0178-2029.
NAL call number: IPS27338
Descriptors: cell culture, roller bottles, in vitro-production, monoclonal antibodies, laboratory scale, foetal, newborn, mouse, donor calf serum, culture, hybridoma, ascitic fluid.

Russell, D.R. (August 21, 1994). The production of industrial and biopharmaceutical proteins in transgenic plants. Abstracts of Papers of the American Chemical Society 208:P56-AGFD, ISSN: 0065-7727.
NAL call number: QD1 C4836 1994
Descriptors: DNA, genetics, corn, human diseases.

Salvo, N.J., A. Castillo, et al. (1994). Optimization of ior-T3 monoclonal antibody production using hollow fiber bioreactors. Biotecnologia Aplicada 11(2):160-164.
Descriptors: cellular density, hybridoma cell line, immunologic method.

Sanchez-Madrid, F. and T.A. Springer (1986). Production of Syrian and Armenian hamster monoclonal antibodies of defined specificity. Methods in Enzymology 121:239-44, ISSN:0076-6879.
NAL call number: QP601 M49
Descriptors: hamsters, monoclonal biosynthesis, hybridomas, tissue culture methods.

Sanders, G.M., J.A. Verschoor, et al. (November 1994). Production of monoclonal antibodies against Xanthomonas campestris pv. mangiferaeindicae and their use to investigate differences in virulence. Journal of Applied Bacteriology 77(5):509-518, ISSN:0021-8847.
NAL call number: 448.39 So12
Descriptors: cell fusion, monoclonal antibodies, plant diseases, mango, Xanthomonas campestris, antibody specificity, epitopes, IgG, Inbred BALB/c mice.

Sandhu, J.S. (December 1994). A rapid procedure for the humanization of monoclonal antibodies. Gene 150(2):409-410, ISSN:0378-1119.
NAL call number: QH442 A1G4
Descriptors:mice, murine monoclonal antibodies, mutagenesis, nucleic acid, heteroduplexes, polymerase chain reaction.

Saxby, S.J.Y. (Autumn 1999). Perspective on in vitro production of monoclonal antibodies. Lab Animal 9-11, ISSN: 0093-7355.
NAL call number: QL55.A1L33
Descriptors: in vitro technologies, monoclonal antibody (MAb) production, ascites method, cell-derived therapeutic products, IACUC responsibility, alternatives, in vivo production.

Schade, R. P. Henklein, et al. (1996). Specificity of chicken (IgY) versus rabbit (IgG) antibodies raised against cholecystokinin octapeptide (CCK-8). Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):80-85, ISSN: 0946-7785.
Descriptors: avian versus mammalian antibodies, radioimmunoassay, Freund's adjuvant.

Schade, R. and A. Hlinak (October 1996) Egg yolk antibodies: state of the art and future prospects. In: Second World Congress on Alternatives and Animal Use in the Life Sciences October 20-24, 1996, Utrecht, The Netherlands, J.B.F. van der Valk and L.F.M. van Zutphen, eds., Alternatives to Laboratory Animals: ATLA 24(Special Issue):120, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: abstract, animal suffering, alternative method, reduction, refinement, replacement, IgY antibodies.

Schade, R. and A. Hlinak (1996). Egg yolk antibodies: state of the art and future prospects. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):5-9, ISSN: 0946-7785.
Descriptors: Ig-Y technology, alternative method, chicken housing, immunization protocol.

Schade, R., C. Pfister, R. Halatsch, and P. Henklein (1991). Polyclonal IgY antibodies from chicken egg yolk: An alternative to the production of mammalian IgG type antibodies in rabbits. Alternative to Laboratory Animals: ATLA 19:403-419, ISSN:0261-1929.
NAL call number: Z7994 L3A5
Descriptors: egg yolk, polyclonal antibodies, pain, immunocytochemistry.

Schäffner, G., M. Haase, and S. Giess (1996). Investigation of the product equivalence of monoclonal antibodies for, therapeutic and in vivo diagnostic human use in case of change from ascitic, to in vitro production. In: Developments in Biological Standardization, Vol. 86: Replacement, Reduction, and Refinement of Animal Experiments in the Development and Control of Biological Products F.K. Brown, K. Cussler and C. Hendriksen, eds., S. Karger: New York, NY, USA, pp. 319-320, ISBN:3-8055-6260-8.
NAL call number: QR180.3 D4 v.86
Descriptors: pharmacological and toxicological evaluation, legal regulations, European Union, clinical trials.

Schelling, M.E. (1995) Methods of immunization to enhance the immune response to specific, antigens in vitro. In: Methods in Molecular Biology: Vol. 45. Monoclonal Antibody Protocols W.C. Davis, ed., Humana Press Inc.: Totowa, NJ, USA, pp. 9-15, ISBN:0-89603-308-2.
NAL call number: QH506 M45 no.45
Descriptors: spleen cells, myeloma cells, B lympocytes, alternative to mouse immunization.

Schlaper, B.S., M. Scheibler, et al. (1995). Development of optimized transfectoma cell lines for production of, chimeric antibodies in hollow fiber cell culture systems. Biotechnology and Bioengineering 45(4):310-319, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: mouse human chimeric antibodies, SP2/0, mouse myeloma cells, conventional culture, hollow fiber system. bioreactor genetic engineering, immunoglobulin expression, growth medium, cones, fermentor.

Schlebusch, H., S. Reinartz, R. Kaiser, et al. (February 1997). Production of a single-chain fragment of the murine anti-idiotypic antibody ACA125 as phage-displayed and soluble antibody by recombinant pha ge antibody technique. Hybridoma 16(1):47-52, ISSN:0272-457X.
NAL call number: QR185.8 H9H9
Descriptors: tumor-associated antigen CA125, vaccine, carcinoma, mouse hybridoma cell line, Escherichia coli TG1 cells, recombinant proteins.

Schniering, A. R. Schade, and T. Hiepe (1996). Development of an IgY based assay for the detection of ascaris suum antigens. [Aufbau eines assays zur detektion von Ascaris summ antigenen auf der basis von IgY.] Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):5-9, ISSN: 0946-7785.
Descriptors: copro antigen, IgY, enzyme immuno assay (EIA).

Schmell, E.D., L.C. Yuan, B.J. Gulyas, and J.T. August (February 1982). Identification of mammalian sperm surface antigens. I. Production of monoclonal anti-mouse sperm antibodies. Fertilization and Sterilization 37(2):249-257, ISSN:0015-0282 .
Descriptors: hybridoma cell lines , mouse myelomat cells, rat spleen cells. C3H mice.

Schmidt, P., M. Erhard (1996). Local reations after the application of several adjuvants in the chicken. [Lokale reaktionen bei verwendung verschiedener adjuvantien bei legehennen.] Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):30-34, ISSN: 0946-7785.
Descriptors: chicken, adjuvants, lipopeptides, Freund's complete adjuvant, muramyl-dipeptide, myositis.

Schmitt, J.J., U. Zimmermann, and G.A. Neil (February 1989). Efficient generation of stable antibody forming hybridoma cells by electrofusion. Hybridoma 8(1):107-115, ISSN:0272-457X.
NAL call number: QR185.8.H9H9
Descriptors: murine hybridomas, electrofusion protocols, temperature, bovine serum albumin.

Schulze, B., C. Middendorf, et al. (1994). Automated imunoanalysis systems for monitoring mammalian cell cultivation processes. Cytotechnology 15(1-3):259-269, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: monoclonal antibody biosynthesis, recombinant proteins, tissue culture methods, biotechnology methods.

Schwarzkopf, C. and B. Thiele (1996). Effectivity of different methods for the extraction and purification of IgY. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):35-39, ISSN: 0946-7785.
Descriptors: IgY, White Leghorn, Freund's, egg yolk, precipitation.

Seamans, T.C. and Q.S. Hu (1990). Kinetics of growth and antibody production by a hybridoma cell line in a perfusion culture. Journal of Fermentation and Bioengineering 70(4):241-245, ISSN: 0922-338X.
NAL call number: QP601 A1J6
Descriptors: cell culture, animal, hybridoma, cell proliferation, antibody, glucose, growth rate, optimization, mixed tank reactor, microfiltration, hollow fiber, perfusion.

Sem, D.S., E.J. Victoria, et al. (1996). Solution structure of a peptide selected from a phage display library using anti-cardiolipin antibody: a beta-2-GPI epitope mimic. FASEB Journal 10(6):A1237, ISSN:0892-6638.
NAL call number: QH301.F3
Descriptors: human, amino acid, hydrogen bonding, peptide shape, carboxyl terminal, glycine, protein structure, systemic lupus erythematosus, recurrent thrombosis.

Shevitz, J., S. Reuveny, T.L. LaPorte, and G.H. Cho (1989). Stirred tank perfusion reactors for cell propagation and monoclonal antibody production. Advances in Biotechnological Processes 11:81-106, ISSN:0736-2293.
NAL call number: TP248.13 A38
Descriptors: perfusion, instrumentation, cell division, cultured cells.

Shueh, L. S. Sitlani, and T. Beck (1993). Monoclonal antibody production by artificial capillary hybridoma culture. Joint Meeting of the American Association of Immunologists and the Clinical Immunology Society May 21-25, 1993. Denver, Colorado. Journal of Immunology 150(8 PART 2):327A, ISSN:0022-1767.
Descriptors: hybridoma culture, pore size, serum concentration, antibody yield.

Simpson, S.D., D.P. Snider, et al. (January 1996). Soluble FcR block suppressor T cell activity at low concentration in, vitro allowing isotype-specific antibody production. Cellular Immunology 167(1):122-128, ISSN:0008-8749.
NAL call number: QR180 C4
Descriptors: mice, Inbred CA, IgA, IgG binding factors, macrophages, T cells, immunoglobulin isotypes, biosynthesis, T-Lymphocytes, lymphokines.

Spicer, S.S., M.A. Spivey, M. Ito, and B.A. Schulte (February 1994). Some ascites monoclonal antibody preparations contain contaminants that bind to selected Golgi zones or mast cells. Journal of Histochemistry and Cytochemistry 42 (2):213-221, ISSN:0022-1554.
NAL call number: 381 J8222
Descriptors: ascitic fluid, Golgi apparatus, immunoenzyme techniques, hybridomas, mice, Inbred BALB/c, Inbred C57BL, rats, Sprague-Dawley.

Spieker-Polet, H., P. Sethupathi, P.C. Yam, and K.L. Knight (September 26,1995). Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas. Proceedings of the National Academy of Sciences 92(20):9348-9352, ISSN:0027-8424.
NAL call number: 500 N21P
Descriptors: plasmacytoma cell lines, spleen cells, hybridomas, monoclonal biosynthesis, blood proteins, cell fusion.

Srikumaran, S., A.J. Guidry, and R.R. Goldsby (March 1984). Production and characterization of monoclonal bovine immunoglobulins G1, G2 and M from bovine x murine hybridomas. Veterinary Immunology and Immunopathology 5(4):323-342, ISSN:0165-2427.
NAL call number: SF757.2 V38
Descriptors: bovine B lymphocytes, murine cell line, cell culture.

Staak, C. (1996). Egg yolk antibodies (IgY) in routine diagnostic work. Alternativen zu Tierexperimenten: ALTEX 13(Supplement 96):73-75, ISSN: 0946-7785.
Descriptors: alternatives to mammalian antibodies, serological diagnosis, zoonoses, animal diseases.

Steineker, F., J. Kreuter, and J. Loewer (1991). High antibody titers in mice with polymethylmethacrylate nanoparticles as an adjuvant for HIV vaccines. Aids 5(4):431-436, ISSN:0954-0121.
Descriptors: comparison, aluminum hydroxide formulations, human immunodeficiency virus.

Stewart-Tull, D. (October 1996) Refinement to the use of adjuvants in the production of polyclonal antibodies. In: Second World Congress on Alternatives and Animal Use in the Life Sciences October 20-24, 1996, Utrecht, The Netherlands, J.B.F. van der Valk and L.F.M. van Zutphen, eds., Alternatives to Laboratory Animals: ATLA 24(Special Issue):118, ISSN:0261-1929.
NAL call number: Z7994.L3A5
Descriptors: abstract, high titers, animal species, route of administration, antigen per dose, cytotoxicity.

Stiftung Forshung 3R (September 1994). MABs Without Mice? 3R-INFO Bulletin 2.
Descriptors: hollow fiber reactors, monoclonal antibody production, culture systems.

Stills, H.F. (1994). Polyclonal antibody production. In: The Biology of the Laboratory Rabbit P.J. Manning, D.H. Ringler and C.E. Newcomber, eds., 2nd edition, Academic Press, Inc., New York, pp. 435-448, ISBN: 0-12-469235-4.
NAL call number: SF966.5 B56 1994
Descriptors: humoral antibody response, routes of injection, necrosis, inflammation, Freunds's adjuvant, Squalene, TiterMax, Ribi, alum, lipopolysaccharides, liposomes, saponins.

Stoll, T., C. Perregaux, U. Von Stockar, I.W. Marison (1995). Production of immunoglobulin A in different reactor configurations. Cytotechnology 17(1):53-63, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: murine hybridoma line (Zac3), T-flask, stirred tank bioreactor, hollow fiber reactor, BALB/c mouse, ascites fluid.

Stoltenborg, J.K., P.W. Tsao, et al. (September 1994). A fluorescent cellular adhesion assay using insect cell produced human VCAM1. Journal of Immunological Methods 175(1):59-68, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: cell adhesion, cell line, flow cytometry, recombinant proteins, tumor cells, cultured.

Takagi, M., K.I. Ohara, and T. Yoshida (1995). Effect of hydrostatic pressure on hybridoma cell metabolism. Journal of Fermentation and Bioengineering 80(6):619-621, ISSN:0922-338X.
NAL call number: QP601 A1J6
Descriptors: mice, cell growth, glucose consumption, Afp-27 cell line, synthetic method, immunological method.

Tarzi, A. and F.B. Orlans (1995). Directory of funding sources for scientific pursuit of alternatives. In: The World Congress on Alternatives and Animal Use in the Life Sciences: Education, Research, Testing, Alternative Methods in Toxicology and the Life Sciences, Volume 11, Mary Ann Liebert, Inc. , New York, NY, pp.703-712, ISBN: 0913113662.
NAL call number: RA1199 A49 v.11 (in progress)
Descriptors: groups that fund alternatives research, some have funded in vitro antibody production methods, industrial, government, foundations, humane societies.

Terashima, S., T. Ogawa, et al. (1993). Immobilization of hybridoma cells with macroporous cellulosic support and improved production of monoclonal antibody. Seibutsu-Kogaku Kaishi 71(3):165-170, ISSN:0919-3758.
NAL call number: QP601 A1N3
Descriptors: monoclonal antibody, polyethyleneimine, perfusion culture, fluidized bed bioreactor.

Theodorou, N.A., P. Easterbrook, M. Tyhurst, and S.L. Howell (1981). Islets of langerhans implanted in diffusion chambers do not initiate antibody production. Transplantation 31(1):89-90, ISSN:0041-1337.
NAL call number: QP89 T73
Descriptors: rat, experimental surgery, homotransplantation, langerhansian islet, immunology, endocrine pancreas, endocrinology.

Thompson, J., and T. Pope (February 16,1996). Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: use of phage display to improve affinity and broaden strain reactivity. Journal of Molecular Biology 256(1):77-88, ISSN:0022-2836.
NAL call number: 442.8 J8224
Descriptors: therapeutic monoclonal antibody, in vitro, HIV antibodies, peptide fragments, affinity genetics, cloning.

Titova, N.G., I.V. Razina, et al. (November-December 1995). The production of hybridomas producing monoclonal antibodies to the causative agent of melioidosis by using antigen-stimulated lymphocytes in an in vitro system. [Poluchenie gibridom-produtsentov monoklonal'nykh antitel k vozbuditeliu melioidoza s ispol'zovaniem limfotsitov stimulirovannykh antigenom v sisteme in vitro.] Zhurnal Mikrobiologii Epidemiologii I Immunobiologii (6):82-83, ISSN:0372-9311.
NAL call number: 448.3 Z4
Descriptors: biosynthesis, monoclonal antibodies, bacterial immunology, Burkholderia pseudomallei, hybridomas immunology, mice, Inbred BALB/c.

Tout, N.L. and J.S. Lam (March 1997). Phage display and bacterial expression of a recombinant Fab specific for Pseudomonas aeruginosa serotype O6 lipopolysaccharide.Clinical and Diagnostic Lab oratory Immunology 4(2):147-155, ISSN:1071-412X .
Descriptors: immunotherapy, antibodies, Pseudomonas aeruginosa, recombinant antibody, filamentous phage.

Trebak, M., J.M. Chong, D. Herlyn, D.W. Speicher (1999). Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology. Journal of Immunological Methods 230(1-2):59-70, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: monoclonal antibodies (Mabs), basic research, cancer therapy, in vitro alternatives, ascites production, laboratory-scale production of Mabs, cultured hybridoma cells, two-chamber cell culture device CELLine 1000, gastrointestinal antigen GA733-2, GA733 MAb and CO17-1AMAb, serum-supplemented medium, serum-free medium (SFM), frequency of nutrient medium changes, cost effective, simple, reproducible, useful alternative.

Tsurushita, N., H. Fu, and C. Warren (June 12, 1996). Phage display vectors for in vivo recombination of immunoglobulin heavy and light chain genes to make large combinatorial libraries. Gene 172(1):59-63, ISSN:0378-1119.
NAL call number: QH442 A1G4
Descriptors: bacteriophage, genetic vectors, immunoglobulins, recombinate genetics, nucleotidyltransferases metabolism.

Tu, L., D. Huang, et al. (1994). Regulatory action of Astragalus saponins and Buzhong Yiqi compound on synthesis of nicotinic acetylcholine receptor antibody in vitro from myasthenia gravis. Chinese Medical Journal 107(4):300-303, ISSN:0366-6999.
Descriptors: saponin, Chinese herb, immunomodulation, Chinese medicine, human cell culture.

Valdes, R., T. Diaz, et al. (1995). Sendai virus removal and inactivation during monoclonal antibody purification. Biotecnologia Aplicada 12(2):115-119. ISSN:0864-4551.
Descriptors: viral contamination, risk, human, ascitic fluid, antibody purification method.

Valdes, V.R. (2002). Alternative techniques to obtain monoclonal antibodies at a small scale: Current state and future goals. Biotecnologia Aplicada 19(3-4):119-131. ISSN:0864-4551.
Descriptors: ascites method, concentrated monoclonal antibodies, alternative methods, summarized, advantages, disadvantages, in vitro techniques, regulatory restrictions, emerging technologies.

Van Der Kamp, M. and W. de Leeuw (October 1996). Short review of in vitro methods for monoclonal antibodies. NCA Newsletter 3:10-12.
Descriptors: Netherlands Centre Alternatives to Animal Use (NCA), spinner flasks, dialysis membrane, roller bottles, hollow fiber, fermentors.

Van Der Pol, J.J., B. Joksch, et al. (1995). On-line control of an immobilized hybridoma culture with multi-channel flow injection analysis. Journal of Biotechnology 43(3):229-242, ISSN:0168-1656.
NAL call number: QH442.J69
Descriptors: glucose, glutamine concentrations, flow injection analysis, specific monoclonal antibody production, lactate, ammonium concentrations, amino acid concentrations.

Van Der Pol, L., G. Zijlstra, M. Thalen, and J. Tramper (1990). Effect of serum concentration of production of monoclonal antibodies and on shear sensitivity of a hybridoma. Bioprocess Engineering 5(6):241-245, ISSN:0178-515X.
NAL call number: TP248.3.B563
Descriptors: concentration, monoclonal antibody, environmental factor, hybridoma, production, bioreactor, continuous stirred tank reactor, cell culture, continuous process, culture medium, animal serum.

Van Wyngaardt, W., D.H. Du Plessis, S. Van Wyngaardt, J.A. Verschoor (June 1992). Production and properties of monoclonal antibodies against African horse sickness virus, serotype 3. Onderstepoort. Journal of Veterinary Research 59(2):129-133, ISSN:0030-2465.
Descriptors: guinea pigs, Inbred BALB/c mice, C57BL polyethylene glycol mediated cell fusions.

Van Der Pol, L., G. Zijlstra, et al. (1990). Effect of serum concentration on production of monoclonal antibodies and on shear sensitivity of a hybridoma. Bioprocess Engineering 5(6):241-246, ISSN:0178-515X.
Descriptors: rat, mouse, cell death rate, serum content, hybridomas, stirred tank reactor.

Vaughan, T.J., A.J. Williams, et al. (1996). Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnology 14(3):309-314, ISSN:1087-0156.
NAL call number: QH442 B5
Descriptors: alternative to hybridoma technology, gene segments, library format, hormones, pharmaceuticals, hybridoma technology, high affinity monoclonal antibodies, genetic engineering, purification method.

Wahl, M.F., G. An, and J.M. Lee (May 1995). Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture. Biotechnology Letters 17(5):463-468, ISSN:0141-5492.
NAL call number: QR53.B56
Descriptors: mice, dimethyl sulfoxide, Nicotiana tabacum, cell suspensions, cell growth, monoclonal antibodies, cell lines.

Wang, G.Z., W.Y. Zhang, D. Freedman, and L. Eppstein (1992). Use of packed bed reactor for production of monoclonal antibodies. Molecular Biology of the Cell 3(Supplement):185A, ISSN:1059-1524.
NAL call number: QH604 C452
Descriptors: abstract, animal hybridoma cells, gamma interferon, immunologic method.

Ward, R.L., M.A. Clark, J. Lees, and N.J. Hawkins (January 16, 1996). Retrieval of human antibodies from phage-display libraries using enzymatic cleavage. Journal of Immunological Methods 189(1):73-82, ISSN:0022-1759.
NAL call number: QR180 J6
Descriptors: human IgG1, kappa gene, pericolic lymph node, bacteriophage M13, recombinant proteins.

WARDS (Summer 1990). Monoclonal antibody production. Science and Animal Care 1(1):1,4
NAL call number: HV4701 S35
Descriptors: animal care, cell culture, monoclonal, ascites, roller culture.

Warren, H.S. and L.A. Chedid (1988). Future prospects for vaccine adjuvants. CRC Critical Reviews in Immunology 8(2):83-101, ISSN:0917-3355.
NAL call number: QR180 C78
Descriptors: aluminum compounds, polyanions, hydrophobic compounds, surfactants, Freund's incomplete adjuvant, liposomes, saponin.

Weichert, H., Falkenberg, F.W., et al. (1995). In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter. In: Alternative Methods in Toxicology and the Life Sciences Series, Vol. 11, Proceedings of the World Congress on Alternatives and Animal Use in the Life Sciences: Education, Research, Testing A.M. Goldberg and L.F. M. van Zutphen, eds., Mary Ann Liebert, Inc.: New York, NY., pp. 253-259, ISBN:0-913-113-66-2.
NAL call number: RA1199 A49
Descriptors: high density culture of hybridoma, dialysis membrane, comparison with in vitro methods.

Weichert, H., M. Krane, et al. (1994). In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter. In Vitro Toxicology 7(2):193, ISSN:0888-319X.
NAL call number: RM300 I55
Descriptors: meeting abstract, mouse, ascitic fluid production, hybridoma, high density cell culture, synthetic method, immunologic method.

Whitelam, G.C., W. Cockburn, and M.R. Owen (November 1994). Antibody production in transgenic plants. Biochemical Society Transactions 22(4):940-944, ISSN:0300-5127.
NAL call number: QD415 A1B58
Descriptors: monoclonal antibody biosynthesis, transgenic biotechnology, gene expression, immunoglobulins, tobacco plant.

Wolff, K.L., B.W. Hudson, R.R. Ormsbee, and M.G. Peacock (October 1976). Production of antibody in induced granulomas. Journal of Clinical Microbiology 4(4):384-387, ISSN:0095-1137.
NAL call number: QR46 J6
Descriptors: granulomas, rabbit, plastic golf balls, cellular migration, titers.

Wongsamuth, R. and P.M. Doran (1996). Production of monoclonal antibody by tobacco hairy roots. 211th American Chemical Society National Meeting, New Orleans, Louisiana, USA, March 24-28, 1996. Abstracts of Papers, American Chemical Society 211 (1-2): BIOT 166, ISSN:0065-7727.
Descriptors: genetic engineering, biotechnology, production levels, bioreactor, clones, transformation, product, yield.

Xie, L. and D.I. Wang (1994). Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15(1-3):17-29, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: nutrient concentrations, toxic byproducts, ammonia, lactate, tissue culture methods, cell line, hybridomas, mice.

Yang, Y.J. and R.F. Coico (February 1996). In vitro enhancement of antibody responses by T delta cells expressing, IgD receptors. Cellular Immunology 168(1):107-116, ISSN:0008-8749.
NAL call number: QR180 C4
Descriptors: murine T cells, adjuvants, antibody producing cells, IgD, T-Lymphocytes, cultured cells.

Yoon, S.J. and K.B. Konstantinov (1994). Continuous, real-time monitoring of the oxygen uptake rate (OUR) in animal cell bioreactors. Biotechnology and Bioengineering 44(8):983-990, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: murine myeloma cell line, monoclonal antibodies, laboratory scale cultivation, industrial scale perfusion culture.

Zamboni, A., I. Giuntini, et al. (1994). Production of mouse monoclonal antibodies using a continuous cell culture fermenter and protein G affinity chromatography. Cytotechnology 16(2): 79-87, ISSN:0920-9069.
NAL call number: QH585.C97
Descriptors: mice, anti-alpha fetoprotein, monoclonal antibodies, double membrane stirrer, electrophoresis, chromatography, hybridoma technology.

Zeng, A.P. (1996). Mathematical modeling and analysis of monoclonal antibody production by hybridoma cells. Biotechnology and Bioengineering 50(3):238-247, ISSN:0006-3592.
NAL call number: 381 J8224
Descriptors: continuous cultures, kinetic model, productivity, nutrients, toxic products, mathematical simulation.

Zeng, Q.S., H. Takeyama, S. Kanda, and R.F. Irie (1994). Serum preparation and methods for the large-scale production of IgG monoclonal antibody. Human Antibodies and Hybridomas (1-2):75-80, ISSN:0956-960X.
Descriptors:fetal calf serum, serum free culture media, mouse myeloma, cell line, cattle, mice, hybridomas.

Zhao, X.(1995). Use of electric field mediated cell fusion to produce hybridomas secreting monoclonal antibodies. Methods in Molecular Biology 45:61-67, ISSN:1064-3745.
NAL call number: QH506.M45
Descriptors: cell fusion, hybridomas, cytology, immunologic techniques, culture media, medical instrumentation, mice, Inbred BALB/c.

Zhou, E., and A. Afshar (1995). Comparison of Freund's adjuvant and TiterMaxTM in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens. Veterinary Immunology and Immunopathology 48(1/2):113-122, ISSN:0165-2427.
NAL call number: SF757.2 V38
Descriptors: Freund's adjuvant, TiterMax, pigs, goats, mice, sequential immunization procedures, viral diseases, immune response, swine diseases.

Zhu, L., F. Guan, W. Mo, et al. (1995). Development of a new anti-CD4 McAb. Zhonghua Weishengquxue He Mianyixue Zazhi 15(1): 32-34, ISSN:0254-5101.
Descriptors: hybridoma, BALB/c mice, recombinant CD4 molecules, epitope recognition, competitive binding test, ascitic fluid.


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